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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa.
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Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa.

机译：结晶和初步的x射线mavicyanin从晶体的研究Cucurbita pepo . medullosa。

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摘要

Mavicyanin isolated from Cucurbita pepo medullosa is a glycosylated protein containing a single polypeptide chain of 109 amino-acid residues and is a member of the phytocyanin subclass of cupredoxins. Non-glycosylated recombinant mavicyanin, which was expressed in Escherichia coli, was crystallized by the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant at pH 5.5. The crystals belonged to the hexagonal space group P6(1) (or P6(5)), with unit-cell parameters a = 64.0, c = 245.0 A, four molecules per asymmetric unit and a solvent content of 59%. X-ray diffraction data were collected to 1.6 A resolution. To solve the structure of mavicyanin, the MAD method as well as a Patterson search method using the structure of stellacyanin as a starting model are presently being utilized.
机译：Mavicyanin隔绝Cucurbita瓠果medullosa是一种糖基化的蛋白包含一个单身吗109个氨基酸残基的多肽链的phytocyanin子类的成员吗cupredoxins。mavicyanin,表达在大肠杆菌、结晶的悬滴vapour-diffusion方法与硫酸铵沉淀剂的pH值5.5。六角空间群P6(1)(或P6 (5)),晶胞参数= 64.0,c = 245.0,每个不对称单元四个分子和溶剂内容的59%。收集到1.6一项决议。mavicyanin结构,疯狂的方法使用结构作为帕特森搜索方法目前stellacyanin作为起始模型被利用。

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