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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of a B-form DNA/RNA chimera (dC)(rG)d(ATCG) complexed with daunomycin at 1.5 A resolution.
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Structure of a B-form DNA/RNA chimera (dC)(rG)d(ATCG) complexed with daunomycin at 1.5 A resolution.

机译：b形式结构DNA / RNA妄想(dC) (rG) d (ATCG)和道诺霉素为1.5一项决议。

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摘要

The crystal structure of a DNA/RNA chimera (dC)(rG)d(ATCG) complexed with the anticancer drug daunomycin has been determined at 1.5 A resolution with R(work) and R(free) of 19.7 and 23.3%, respectively, for 2767 reflections. The complex crystallizes in space group P4(1)2(1)2, with unit-cell parameters a = b = 28.05, c = 53.16 A, and contains one nucleic acid strand and one daunomycin molecule in the asymmetric unit. To our knowledge, this is the first crystal structure of a DNA/RNA chimera complexed with an intercalating drug. The DNA/RNA chimera adopts the B-form helical conformation, with the 2'-hydroxyl group in the major groove of the duplex, forming hydrogen bonds to N7 and the anionic phosphate oxygen of its 3'-side adenine. The present results indicate that the replacement by the ribose sugar in the DNA sequence does not change the geometry and intercalation pattern of daunomycin. A model of B-form RNA has been built based on the present structure. The model indicates that the interactions of the 2'-hydroxyl groups in the B-form duplex depend on their 3'-side nucleotides.
机译：晶体结构的DNA / RNA妄想(dC) (rG) d (ATCG)包裹着抗癌道诺霉素药物已被确定为1.5分辨率与R(工作)和19.7(免费)23.3%, 2767年分别反映。复杂的结晶在空间群P4 (1) 2 (1) 2,晶胞参数a = b = 28.05, c =53.16,包含一个核酸链不对称正定霉素分子之一。据我们所知,这是第一个水晶DNA / RNA嵌合体的结构复杂化的置入药物。b形式螺旋构象,2的羟基组的大沟双,形成氢键N7和3 '端腺嘌呤磷酸阴离子氧气。目前的结果表明,更换糖核糖的DNA序列没有变化的几何和夹层模式道诺霉素。基于目前的结构。表明的交互2》在b形式双工取决于羟基组3 '端核苷酸。

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