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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.
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Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.

机译：初步的x光特性和分阶段的II型的cellulosome cohesin域Acetivibrio cellulolyticus。

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摘要

The N-terminal type II cohesin from the cellulosomal ScaB subunit of Acetivibrio cellulolyticus was crystallized in two different crystal systems: orthorhombic (space group P2(1)2(1)2(1)), with unit-cell parameters a = 37.455, b = 55.780, c = 87.912 A, and trigonal (space group P3(1)21), with unit-cell parameters a = 55.088, b = 55.088, c = 112.553 A. The two crystals diffracted to 1.2 and 1.9 A, respectively. A selenomethionine derivative was also crystallized and exhibited trigonal symmetry (space group P3(1)21), with unit-cell parameters a = 55.281, b = 55.281, c = 112.449 A and a diffraction limit of 1.97 A. Initial phasing of the trigonal crystals was successfully performed by the SIRAS method using Cu Kalpha radiation with the selenomethionine derivative as a heavy-atom derivative. The structure of the orthorhombic crystal form was solved by molecular replacement using the coordinates of the trigonal form.
机译：氨基二型cohesin从Acetivibrio cellulosomal痂子单元cellulolyticus是两个不同的结晶水晶系统:斜方晶系的(空间群P2(1) 2(1) 2(1)),晶胞参数=37.455, b = 55.780, c = 87.912,三方(空间群P3(1) 21),与晶胞参数= 55.088, b = 55.088, c = 112.553。晶体衍射,1.2和1.9,分别。结晶和三角对称展出(空间群P3(1) 21),与晶胞参数b = 55.281, = 55.281和112.449 c =1.97的衍射极限。三方晶系的晶体已成功执行SIRAS方法使用铜Kalpha辐射与硒代蛋氨酸导数重原子衍生物。斜方晶系的分子晶体形式解决更换使用三角形的坐标的形式。

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