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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray crystallographic studies of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum.
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Crystallization and preliminary X-ray crystallographic studies of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum.

机译:结晶和初步的x射线晶体磷酸烯醇丙酮酸的研究从棒状杆菌carboxykinase glutamicum。

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摘要

Phosphoenolpyruvate carboxykinase (PCK) is a key enzyme involved in the regulation of gluconeogenesis. PCKs from higher animals require guanosine nucleotide for activity. PCK from Corynebacterium glutamicum is also GTP specific. X-ray diffraction data from a C. glutamicum PCK crystal were collected to 2.8 A resolution. The crystals were monoclinic, belonging to space group P2(1), with unit-cell parameters a = 71.7, b = 117.4, c = 161.3 A, beta = 92.9 degrees. The presence of two molecules in the crystallographic asymmetric unit gives a V(M) of 2.5 A(3) Da(-1) and a solvent content of 50.3%.
机译:磷酸烯醇丙酮酸carboxykinase (PCK)是一个关键酶参与的规定糖质新生。鸟嘌呤核苷核苷酸的活动。棒状杆菌属glutamicum也是三磷酸鸟苷具体。从c x射线衍射数据glutamicum PCK水晶收集到2.8一项决议。单斜晶体,属于空间集团P2(1),晶胞参数= 71.7,b = 117.4, c = 161.3,β= 92.9度。两个分子在晶体的存在不对称单位给出了V (M)(3)达2.5 (1)和溶剂含量的50.3%。

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