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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.
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Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.

机译：结晶的chaperonin GroEL-GroES从栖热菌属复杂的酸奶HB8。

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摘要

The chaperonin GroEL-GroES (GroEL/ES) complex from a thermophilic eubacteria, Thermus thermophilus HB8, has been purified and crystallized. The GroEL/ES complex is known to be composed of 14 identical GroEL subunits (58 kDa) and seven identical GroES subunits (11 kDa). The GroEL/ES complex crystals belong to the triclinic space group P1, with unit-cell parameters a = 140.4, b = 156.4, c = 273.1 A, alpha = 82.9, beta = 85.4, gamma = 68.5 degrees. The crystal asymmetric unit contains two molecules (MW = 885 kDa). One data set to 3.0 A resolution, with 383 652 independent observations (89.3% complete) and an R(merge) of 0.08, has been collected from a single crystal. A molecular-replacement solution was obtained using the structure of the GroEL/ES complex from Escherichia coli as a search model.
机译：复杂的chaperonin GroEL-GroES (GroEL / ES)嗜热真细菌,栖热菌属酸奶HB8,纯化和结晶。GroEL / ES组成的复杂是14相同GroEL子单元(58 kDa)和7相同的gro子单元(kDa 11日)。复杂的晶体属于三斜晶系的空间群体P1,晶胞参数= 140.4,b= 156.4, c = 273.1,α= 82.9,β= 85.4,γ= 68.5度。包含两个分子(MW = 885 kDa)。设置为3.0一项决议,652年与383年独立观察(89.3%完成)和一个R(合并)0.08,已经收集了从单个晶体。molecular-replacement的解决方案是使用获得的GroEL / ES复杂的结构大肠杆菌作为搜索模型。

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