...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein.
【24h】

Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein.

机译：SARS冠状病毒的结构基因组学:克隆、表达、结晶Nsp9初步晶体研究蛋白质。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family and its genome differs substantially from those of other known coronaviruses. SARS-CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS-CoV whole-genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20-30 mature proteins. The functions of many of these polypeptides, including the Nsp9-Nsp10 replicase-cleavage products, are still unknown. Here, the cloning, Escherichia coli expression, purification and crystallization of the SARS-CoV Nsp9 protein, the first SARS-CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 A resolution and belong to space group P6(1/5)22, with unit-cell parameters a = b = 89.7, c = 136.7 A. With two molecules in the asymmetric unit, the solvent content is 60% (V(M) = 3.1 A(3) Da(-1)).
机译：最近流行的aetiologic代理严重急性呼吸系统综合症(SARS)positive-stranded RNA病毒(冠)归属感Coronaviridae家族及其基因组大大不同于其他已知的冠状病毒。由呼吸路线和日期没有疫苗和预防或治疗对这个代理治疗。开发了针对全基因组方法确定所有的晶体结构蛋白质或域。极大地促进药物设计。冠状病毒在27和31.5 kbp之间长度,已知的最大的RNA病毒,编码20 - 30成熟的蛋白质。这些多肽,包括Nsp9-Nsp10 replicase-cleavage产品,仍然是未知的。的表达、纯化和结晶的冠Nsp9蛋白质,第一冠蛋白质结晶,报道。晶体衍射分辨率和属于2.8空间群P6(1/5) 22日,晶胞参数a = b = 89.7, c = 136.7。分子不对称单位,溶剂内容是60% (V (M) = 3.1 (3) Da(1))。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号