Crystallization and structure analysis of Thermus flavus 5S rRNA helix B.

Vallazza M; Banumathi S; Perbandt MMoore KDeLucas LBetzel CErdmann VA

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and structure analysis of Thermus flavus 5S rRNA helix B.

【24h】

Crystallization and structure analysis of Thermus flavus 5S rRNA helix B.

机译：栖热菌属的结晶和结构分析flavus 5 s rRNA螺旋B。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The crystallization conditions of the synthetic RNA duplex r(GCGGCGU)*r(GCGCCGC), part of the Thermus flavus 5S rRNA domain B, were investigated in detail. The crystallization analysis revealed a relative narrow crystallization zone. Single sequence variations did not enhance the crystal quality, however the crystallization under microgravity provided crystals of higher quality. They belong to the space group P3(1)21 with unit cell dimensions of a = b = 35.0 A and c = 141.2 A. Diffraction data up to 2.6 A were collected and the structure subsequently analysed and refined to an R-value of 22.4 %. The conformation of the two molecules in the asymmetric unit is stabilized by intermolecular hydrogen bonds. The two molecules A and B are perpendicular to each other and interacting head to tail with symmetry related molecules. They form pseudo-continuous infinite helices in the crystal lattice.

机译：合成的结晶条件RNA双r (GCGGCGU) * (GCGCCGC)的一部分栖热菌属flavus 5 s rRNA域B详细调查。分析显示一个相对狭窄结晶区。没有提高晶体质量,然而,结晶在微重力下提供晶体质量高。空间群P3(1) 21单胞尺寸的a = b = 35.0 a和c = 141.2。2.6收集和结构随后分析了热阻和细化的22.4%。在不对称单位是稳定的分子间氢键。A和B是互相垂直头到尾互动与对称性有关分子。螺旋线的晶格。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1700-1703|共4页
作者
Vallazza M; Banumathi S; Perbandt MMoore KDeLucas LBetzel CErdmann VA;
展开▼
作者单位

Freie Universitat Berlin, FB Biologie, Chemie, Pharmazie, Institut fur Chemie,Thielallee 63, 14195 Berlin, Germany.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
crystal lattices; structured analysis; HEAD-TO-TAILThermus thermophilusRibosomesRN5S1@ gene clusterThermusRNACrystallization;

机译：水晶晶格;结构化分析;HEAD-TO-TAILThermusclusterThermusRNACrystallization;

相似文献

外文文献
中文文献
专利

1. Middle or low water pressure direct spiral double helix converging nozzle structure optimization and flow field analysis [J] . Jiang Lin, Wu Ruohn, Zhao Hui, 高技术通讯（英文版） . 2015,第003期
2. High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors [J] . SONG Yang, LIU Hong-di, ZHOU Qiang, 农业科学学报（英文版） . 2017,第003期
3. High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors [J] . SONG Yang, LIU Hong-di, ZHOU Qiang, 农业科学学报：英文版 . 2017,第003期
4. The Thermus thermophilus 5S rRNA -Protein Complex: Identification of Specific Binding Sites for Proteins L5 and L18 in the 5S rRNA [J] . G.M.Gongadze, Institute of Protein Research Russian Academy of Sciences Pushchino Moscow Region 142290 Russia, A.A.Perederina, Molecular Biology . 2001,第4期

机译：Thermus thermophilus 5S rRNA-蛋白质复合物：5S rRNA中蛋白质L5和L18的特异性结合位点的鉴定
5. МИЖВИДОВИЙ ПОЛИМОРФИЗМ И МИНЛИВИСТЬ ГЕНИВ 18S-25S ТА 5S рРНК В КУЛЬТУРИ ТКАНИН RAUWOLFIA BENTH. I GENTIANA L. [J] . В.А. КУНАХ, И.О. АНДРЭЭВ, К.В. СПИРИДОНОВА Физиология и биохимия культурных растений: Науч.- теорет. журн. . 2006,第2期

机译：18秒-25s和5S rRNA在组织中的留下纪念和矿业生成术中的念珠菌和5S rRNA。我是Gentiana L.
6. Glycerol transport and phosphoenolpyruvate-dependent enzyme I- and HPr-catalysed phosphorylation of glycerol kinase in Thermus flavus [J] . Emmanuelle Darbon, Kiyoshi Ito, Hua-Shan Huang, Microbiology . 1999,第11期

机译：甘油运输和磷酸丙酮酸依赖性酶I-和HPR催化的甘油激酶磷酸化在Thermus flavus中
7. Semantic Analysis of 'V+死+人' Structure [C] . Yong Lu, Pengyuan Liu Chinese lexical semantics workshop . 2015

机译：Semantic Analysis of 'V+死+人' Structure
8. Study of common Vibrio 5S ribosomal RNA sequence variants and development of a new methodology to identify the phenotypes of Vibrio proteolyticus 5S rRNA variants. [D] . Zhang, Zhengdong. 2002

机译：常见的弧菌5S核糖体RNA序列变异体的研究和一种新方法的开发，以鉴定蛋白水解弧菌5S rRNA变异体的表型。
9. Sequences of the 5S rRNAs of the thermo-acidophilic archaebacterium Sulfolobus solfataricus (Caldariella acidophila) and the thermophilic eubacteria Bacillus acidocaldarius and Thermus aquaticus. [O] . E Dams, P Londei, P Cammarano, 1983

机译：嗜热嗜酸性古生菌Sulfolobus solfataricus（嗜酸Caldariella acid）和嗜热真细菌嗜酸芽孢杆菌Bacillus acidocaldarius和Thermus aquaticus的5S rRNA序列。
10. Crystallization and X-ray diffraction data of Thermus flavus 5S rRNA helices [O] . Marco Vallazzaa, Andrea Sengea, Markus Perb, 2015

机译：Thermus flavus 5s rRNa螺旋的结晶和X射线衍射数据
11. 2017 Helix Technical Report SERC 2018 TR 101 B. [R] . Hutchinson, N., Verma, D., Burke, P., 2018

机译：2017 Helix技术报告sERC 2018 TR 101 B.

Crystallization and structure analysis of Thermus flavus 5S rRNA helix B.

摘要

著录项

相似文献

相关主题

期刊订阅