Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction.

Berisio R; Vitagliano L; Vergara ASorrentino GMazzarella LZagari A

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Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction.

机译：collagen-like多肽的结晶(PPG) 10国际空间站上。2. 衍射。

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Crystals of the collagen-like polypeptide (PPG)(10) were obtained within the Advanced Protein Crystallization Facility on board the International Space Station, during the STS-105/STS-108 mission. The duration of this mission was such to ensure that the crystallization process had reached its end. Crystals were grown both in the presence and in the absence of agarose gel, to compare the quality of the crystals obtained from these different environments. As a result, crystals grown in the absence of agarose on Earth as well as in microgravity showed X-ray diffraction up to 1.15 A. The intensity/sigma ratio was slightly higher for microgravity grown crystals. Crystals grown in agarose gel, both in microgravity and on ground, showed a comparable diffraction power, with a resolution limit of 1.45 A.

机译：collagen-like多肽的晶体(PPG)(10)中获得先进蛋白质结晶设备上国际空间站,在sts - 105 / sts - 108任务。的任务是确保结晶过程已经结束了。晶体都在存在和成长琼脂糖凝胶的缺席,比较从这些获得的晶体质量不同的环境。生长在地球上没有琼脂糖在微重力显示x射线衍射1.15。高微重力晶体生长。生长在琼脂糖凝胶,在微重力和地面,相对衍射显示权力,1.45分辨率的限制。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1695-1699|共5页
作者
Berisio R; Vitagliano L; Vergara ASorrentino GMazzarella LZagari A;
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作者单位

Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 6, I-80134, Napoli, Italy.;

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正文语种英语
中图分类化学;
关键词
Sepharose; Microgravity; CollagenWeightlessnesscrystalsX-ray diffractionPolypeptidesAgarose GelPeptidescrystallization processCrystallizationresolution limit;

Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction.

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