Separating nucleation and growth in protein crystallization using dynamic light scattering.

Saridakis E; Dierks K; Moreno ADieckmann MWChayen NE

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Separating nucleation and growth in protein crystallization using dynamic light scattering.

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Separating nucleation and growth in protein crystallization using dynamic light scattering.

机译：分离成核和生长的蛋白质用动态光散射结晶。

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A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.

机译：控制结晶的一种手段单独的成核和生长的阶段。方法来实现这一目标,除了播种,包括降低过度饱和的变化温度或稀释后滴对于一个给定的时间在成核孵化条件。晶体在显微镜下可见许多核形成。散射实际应用,确定nucleation-growth最有可能的时间解耦成功执行。DLS显示显著改变大小分布的物种的解决方案,对应的最佳时间。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1597-1600|共4页
作者
Saridakis E; Dierks K; Moreno ADieckmann MWChayen NE;
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作者单位

Biological Structure and Function Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, UK.;

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正文语种英语
中图分类化学;
关键词
SEPARATION PROCESSES; Supersaturation; CONTROLLED CRYSTALLISATIONDynamic Light Scatteringphoton correlation spectroscopyNUCLEATseed inoculationCrystallizationLight scattering;

机译：分离过程;过度饱和的控制CRYSTALLISATIONDynamic光Scatteringphoton相关spectroscopyNUCLEATseedinoculationCrystallizationLight散射;

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Separating nucleation and growth in protein crystallization using dynamic light scattering.

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