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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Two polymorphs of lysozyme nitrate: temperature dependence of their solubility.
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Two polymorphs of lysozyme nitrate: temperature dependence of their solubility.

机译:两种硝酸溶菌酶的多晶型物:温度依赖他们的溶解度。

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Two crystallographic forms of lysozyme nitrate are known, namely monoclinic and triclinic. Having previously determined the temperature dependence of the solubility of the monoclinic form (0.2 M NaNO (3) solutions at pH = 4.5) [Legrand et al. (2001). J. Crystal Growth 232, 244-249], we focus here on the solubility of the triclinic form. The temperature dependence of the solubility of this crystallographic form has been measured with a static light device developed in our laboratory. This device allows to observe of the dissolution of one phase and/or the occurrence of a new one by varying the temperature with a sweep rate as low as 0.6 degree/hour. The new solubility data are complemented with crystallographic data of the triclinic form for the sake of completeness. The faces of a triclinic crystal are indexed. The crystallisation enthalpy of the triclinic form is deduced from these new results. These new solubility data allow us now to discuss (1). the published protocols used to obtain the monoclinicand triclinic forms of lysozyme nitrate and (2). the phase transformation.
机译:两个晶体形式的硝酸溶菌酶众所周知,即单斜、三斜晶系的。先前确定的温度依赖性溶解度的单斜形态(0.2 M纳米(3)解决方案在pH = 4.5) [Legrand et al。(2001)。这里的溶解度三斜晶系的形式。温度依赖的溶解度测量了晶体形式静态光设备在我们实验室开发的。这个设备可以观察的解散一阶段和/或一个新的的发生通过改变温度与扫描速度低至0.6度/小时。辅以晶体数据的吗三斜晶系的形式的完整性。三斜晶系的晶体的面孔是索引。三斜晶系的结晶焓的形式从这些新结果推导出。溶解度数据让我们现在讨论(1)。协议用于获得发表monoclinicand三斜晶系的形式的硝酸溶菌酶和(2)阶段的转换。

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