BPTI liquid-liquid phase separation monitored by light and small angle X-ray scattering.

Grouazel S; Perez J; Astier JPBonnete FVeesler S

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BPTI liquid-liquid phase separation monitored by light and small angle X-ray scattering.

机译：BPTI液-液分离监控阶段光和小角x射线散射。

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In the field of protein crystallization, a better knowledge of the nucleation process is essential to control the nucleation rate, the growth and therefore the size and the quality of crystals. With that aim, it becomes clear that the important stage is the determination of the protein phase diagram. We highlighted and investigated the bovine pancreatic trypsin inhibitor (BPTI) binary liquid-liquid phase separation in 350 mM KSCN solutions as a function of temperature. We measured the low concentration part of the binodal curve using light scattering and optical microscopy. We show, from small angle X-ray scattering experiments, that the high concentrated phase sediments in the bottom of the capillary and we analysed the low concentrated phase in terms of monomers/decamers equilibrium.

机译：领域的蛋白质结晶,更好成核过程的知识是至关重要的控制成核率,生长因此晶体的大小和质量。目标,它变得清楚重要阶段的决心蛋白质的相图。调查了牛胰腺胰蛋白酶抑制剂(BPTI)二元液-液阶段分离在350毫米KSCN作为函数的解决方案的温度。使用光散射双结点曲线的一部分和光学显微镜。x射线散射实验中,高集中相沉积物的底部毛细管和我们分析了低集中阶段的单体/ decamers平衡。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1560-1563|共4页
作者
Grouazel S; Perez J; Astier JPBonnete FVeesler S;
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作者单位

Centre de Recherche sur les Mecanismes de la Croissance Cristalline, CRMC2#-CNRS, Campus de Luminy, Case 913, F-13288 Marseille cedex 09, France.;

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正文语种英语
中图分类化学;
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Aprotinin; function of temperature; binodal curvenucleation rateSmall angle X ray scatteringPhase separationoptical microscopyX ray scatteringLight scattering;

机译：抑肽酶;温度的函数;双结点curvenucleation rateSmall角X射线scatteringPhase separationoptical microscopyX雷scatteringLight散射;

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BPTI liquid-liquid phase separation monitored by light and small angle X-ray scattering.

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