Automated mounting, centering and screening of crystals for high-throughput protein crystallography.

Karain WI; Bourenkov GP; Blume HBartunik HD

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Automated mounting, centering and screening of crystals for high-throughput protein crystallography.

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Automated mounting, centering and screening of crystals for high-throughput protein crystallography.

机译：自动安装,定心和筛选晶体的高通量蛋白质晶体学。

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A fully automated system for screening protein crystals for X-ray diffraction analysis has been designed and is being installed on the beamline BW6 at DORIS in Hamburg, Germany. The system includes robotic mounting of flash-frozen crystals from a storage dewar, centering and alignment of the sample both by optical and X-ray (scattering and fluorescence) techniques, assessment of the diffraction quality of the sample, and SAD/MAD or non-conventional diffraction data acquisition with high-throughput data rates. The system covers all experimental steps required for protein x-ray structure analysis and provides a powerful means for structural genomics projects.

机译：一个完全自动化的系统筛选蛋白质晶体x射线衍射分析设计和安装在beamlineBW6多丽丝在德国汉堡。包括瞬间冷冻的机械安装晶体从存储杜瓦,定心对齐的样本通过光学和x射线(散射和荧光)技术,的衍射质量的评估样本,悲伤/疯狂的或非常规与高通量衍射数据采集数据率。蛋白质x射线结构所需的步骤分析和提供了一个强有力的手段结构基因组学项目。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1519-1522|共4页
作者
Karain WI; Bourenkov GP; Blume HBartunik HD;
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作者单位

Max Planck Research Unit for Structural Molecular Biology, MPG-ASMB c/o DESY, Notkestrasse 85, Hamburg, Germany.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
crystals; functional/structural genomics; CrystallographyAlignmentfully automated systemX-ray diffraction analysisX ray scattering;

机译：晶体;功能/结构基因组学;CrystallographyAlignmentfully自动化systemX-ray衍射analysisX射线散射;

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Automated mounting, centering and screening of crystals for high-throughput protein crystallography.

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