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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of bucain, a novel toxin from the Malayan krait Bungarus candidus.
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Crystallization and preliminary X-ray analysis of bucain, a novel toxin from the Malayan krait Bungarus candidus.

机译：结晶和初步的x射线分析bucain,小说从马来亚金环蛇毒素Bungarus candidus。

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摘要

Bucain is a three-finger toxin, structurally homologous to snake-venom muscarinic toxins, from the venom of the Malayan krait Bungarus candidus. These proteins have molecular masses of approximately 6000-8000 Da and encompass the potent curaremimetic neurotoxins which confer lethality to Elapidae and Hydrophidae venoms. Bucain was crystallized in two crystal forms by the hanging-drop vapour-diffusion technique in 0.1 M sodium citrate pH 5.6, 15% PEG 4000 and 0.15 M ammonium acetate. Form I crystals belong to the monoclinic system space group C2, with unit-cell parameters a = 93.73, b = 49.02, c = 74.09 A, beta = 111.32 degrees, and diffract to a nominal resolution of 1.61 A. Form II crystals also belong to the space group C2, with unit-cell parameters a = 165.04, b = 49.44, c = 127.60 A, beta = 125.55 degrees, and diffract to a nominal resolution of 2.78 A. The self-rotation function indicates the presence of four and eight molecules in the crystallographic asymmetric unit of the form I and form II crystals, respectively. Attempts to solve these structures by molecular-replacement methods have not been successful and a heavy-atom derivative search has been initiated.
机译：Bucain三指毒素,结构同源,蛇毒毒蕈碱的毒素,马来金环蛇的毒液Bungarus。candidus演变来这些蛋白质的分子质量大约6000 - 8000 Da和包括强有力的curaremimetic带来的神经毒素杀伤力眼镜蛇科和Hydrophidae毒液。Bucain在两种晶体形式的结晶的悬滴vapour-diffusion技术0.1 M柠檬酸钠pH值为5.6,15% 4000年和挂钩0.15醋酸铵。单斜晶系,空间群C2晶胞参数= 93.73,b = 49.02, c =74.09,β= 111.32度,和衍射名义1.61解决。也属于空间群C2,晶胞参数= 165.04,= 49.44 b, c = 127.60,β= 125.55度,衍射名义2.78解决。表示4和8的存在晶体中的分子不对称单位表单的I和II晶体形式,分别。试图解决这些结构molecular-replacement没有方法成功的和重原子衍生物搜索被启动。

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