Expression, purification, crystallization and preliminary X-ray analysis of the sexual stage-specific protein Pfg27 from Plasmodium falciparum.

Kumar Singh S; Prasad Sati S; Kongkasuriyachai DKumar NSharma A

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification, crystallization and preliminary X-ray analysis of the sexual stage-specific protein Pfg27 from Plasmodium falciparum.

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Expression, purification, crystallization and preliminary X-ray analysis of the sexual stage-specific protein Pfg27 from Plasmodium falciparum.

机译：表达,纯化,结晶初步的x射线分析性stage-specific蛋白质Pfg27疟原虫恶性疟原虫。

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The differentiation and development of sexual stages in Plasmodium falciparum is a complex process which involves the expression of several sexual stage-specific proteins. Pfg27 is one of the most crucial proteins and is expressed abundantly at the onset of gametocytogenesis. An expression and purification system for Pfg27 has been established that yields approximately 5 mg l(-1) of purified protein in a soluble form. This protein has been crystallized by the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant. The original crystal size was improved significantly by the addition of glucose to the reservoir solution. Pfg27 crystals belong to the space group C222(1), with unit-cell parameters a = 58.9, b = 113.2, c = 91.6 A. Native diffraction data were collected under cryogenic conditions and phase resolution by a selenomethionine-aided multiple-wavelength anomalous dispersion technique is in progress. The Pfg27 structure will provide a framework for functional and biochemical studies aimed at understanding gametocyte development in P. falciparum.

机译：性的分化和发育在恶性疟原虫是一个复杂的阶段过程包括几个的表达性stage-specific蛋白质。最重要的蛋白质和表达大量gametocytogenesis发作。Pfg27表达和净化系统建立了收益率大约5毫克l(1)纯化蛋白的可溶性形式。蛋白质结晶的悬滴vapour-diffusion方法使用8000作为一个挂钩沉淀剂。明显改善的葡萄糖水库的解决方案。的空间群C222(1),与单胞参数= 58.9,b = 113.2, c = 91.6。本地衍射数据收集低温条件和相位分辨率selenomethionine-aided多波长反常色散的技术进步。Pfg27结构将提供一个框架针对功能和生化研究理解配子体发育P。恶性疟原虫。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|1868-1870|共3页
作者
Kumar Singh S; Prasad Sati S; Kongkasuriyachai DKumar NSharma A;
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Malaria Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.;

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正文语种英语
中图分类化学;
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phase resolution; X-ray analysis; Plasmodium falciparumspace groupsProtozoan Proteinspurification systems;

机译：相位分辨率、x射线分析、疟原虫falciparumspace groupsProtozoanProteinspurification系统;

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Expression, purification, crystallization and preliminary X-ray analysis of the sexual stage-specific protein Pfg27 from Plasmodium falciparum.

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