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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray characterization of archaeal group II chaperonin alpha-subunit from Thermococcus strain KS-1.
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Crystallization and preliminary X-ray characterization of archaeal group II chaperonin alpha-subunit from Thermococcus strain KS-1.

机译：结晶和初步的x射线描述的热点组II chaperonin从Thermococcus应变KS-1 alpha-subunit。

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摘要

The archaeal group II chaperonin from Thermococcus strain KS-1 is composed of two kinds of subunits (alpha and beta). Each of the recombinant subunits was individually expressed in Escherichia coli and purified as homo-hexadecamers of each subunit. Both homo-oligomers facilitate the refolding of denatured proteins in vitro in an ATP-dependent manner. A mutant alpha-subunit homo-oligomer with two amino-acid substitutions, which has the ability to capture the unfolded protein but lacks the ability to refold the unfolded protein, was crystallized in two different conditions. One crystal form was obtained from a high-concentration solution of ammonium sulfate and grew to maximum dimensions of 0.15 x 0.15 x 0.4 mm. The crystals of this form belonged to the tetragonal space group P42(1)2, with unit-cell parameters a = b = 209.3, c = 156.1 A, and diffracted X-rays to 2.4 A resolution with synchrotron radiation. The other form was crystallized from a polyethylene glycol 6000 solution and belonged to the tetragonal space group, with unit-cell parameters a = b = 220.8, c = 182.4 A. This form only diffracts X-rays to 6 A resolution. Diffraction data collected from the former crystal enabled initial successful phases to be obtained by the molecular-replacement method.
机译：古细菌组II chaperonin Thermococcus应变KS-1两种亚基组成(α和β)。子单元单独表达大肠杆菌和纯化每个亚基的homo-hexadecamers。homo-oligomers促进重折叠的变性蛋白质体外ATP-dependent的方式。有两个氨基酸替换捕获的蛋白质,但缺乏能力折叠的蛋白质的能力,在两种不同条件下结晶。水晶形式来自于一位高浓度的硫酸铵溶液和增长的最大尺寸0.15 x 0.15 x0.4毫米。正方空间群第42页(1)2、晶胞参数a = b = 209.3, c = 156.1,x射线衍射分辨率2.4同步加速器辐射。从聚乙二醇6000结晶解决方案和属于正方空间集团与晶胞参数a = b = 220.8, c= 182.4。决议。启用前水晶最初成功的阶段molecular-replacement获得的方法。

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