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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori.
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Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori.

机译：结晶和初步的x射线晶体enoyl-acyl载体的分析从幽门螺旋杆菌蛋白还原酶。

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摘要

Enoyl-acyl carrier protein reductase (ENR) catalyzes the NADH-dependent stereospecific reduction of alpha,beta-unsaturated fatty acids bound to the acyl-carrier protein. ENR from Helicobacter pylori has been overexpressed in Escherichia coli and has been crystallized in the presence of its cofactor NADH and the inhibitor triclosan (or its analogue diclosan) at 296 K using polyethylene glycol (PEG) 400 as a precipitant. For the triclosan (or diclosan) complex, diffraction data to 2.5 (or 2.3) A resolution have been collected using synchrotron X-rays. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 73.35, b = 94.91, c = 75.38 A, beta = 106.21 degrees for the triclosan complex (or a = 73.25, b = 95.07, c = 75.02 A, beta = 106.53 degrees for the diclosan complex). The asymmetric unit contains one homotetramer, with a corresponding V(M) of 2.10 A(3) Da(-1) and a solvent content of 41% by volume.
机译：Enoyl-acyl载体蛋白还原酶(ENR)催化NADH-dependent立体定向减少α,beta-unsaturated脂肪酸绑定到酰基载体蛋白。幽门螺杆菌在大肠杆菌和结晶的代数余子式NADH和抑制剂的存在三氯生在296 K(或其模拟diclosan)用聚乙二醇(PEG)的400年沉淀剂。复杂的衍射数据2.5(或2.3)的风险使用同步加速器决议已经收集了x射线。空间群P2(1),晶胞参数=73.35, b = 94.91, c = 75.38,β= 106.21度的三氯生复杂(或= 73.25,b = 95.07, c = 75.02,β= 106.53度diclosan复杂)。包含一个homotetramer,相应(3) 2.10 V (M)的Da(1)和溶剂的含量体积的41%。

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