Crystallization and preliminary crystallographic studies on the chromo shadow domain (CSD) of mouse heterochromatin protein M31.

Gao Y; Ding Y; Singh PBRao Z

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary crystallographic studies on the chromo shadow domain (CSD) of mouse heterochromatin protein M31.

【24h】

Crystallization and preliminary crystallographic studies on the chromo shadow domain (CSD) of mouse heterochromatin protein M31.

机译：结晶和初步的晶体研究彩色影子域(CSD)鼠标M31异染色质蛋白质。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Members of the heterochromatin protein 1 (HP1) class of non-histone chromosomal proteins are components of heterochromatin and are involved in the epigenetic regulation of the genome. HP1 proteins are modular and consist of two sequence-related domains called the chromodomain (CD) and the chromo shadow domain (CSD). In order to investigate the role of the murine HP1-like protein M31 in heterochromatin formation and gene silencing, recombinant CSD was overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method with PEG 4000 as precipitant. Diffraction data to 2.9 A were collected from a native crystal belonging to space group C222(1), with unit-cell parameters a = 60.0, b = 95.6, c = 91.7 A, alpha = beta = gamma = 90 degrees.

机译：成员的异染色质蛋白1 (HP1)类non-histone染色体的蛋白质组件的异染色质和参与基因组的表观遗传调控。蛋白质是模块化和由两个称为chromodomain sequence-related域(CD)和色域阴影(CSD)。调查小鼠HP1-like的角色蛋白质M31异染色质形成和基因沉默,重组CSD是过表达大肠杆菌和固定使用悬滴vapour-diffusion方法挂钩4000年作为沉淀剂。从原生晶体属于收集空间群C222(1),一个晶胞参数= 60.0, b = 95.6, c = 91.7,α=β=γ= 90度。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|1051-1053|共3页
作者
Gao Y; Ding Y; Singh PBRao Z;
展开▼
作者单位

Laboratory of Structural Biology and MOE Laboratory of Protein Science School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
Heterochromatin; Non-Histone Chromosomal Proteins; CBX1 geneshadowsCBX5 geneGene SilencingCrystallization;

机译：异染色质;Non-Histone染色体蛋白质;CBX1 geneshadowsCBX5 geneGeneSilencingCrystallization;

相似文献

外文文献
中文文献

1. Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein [O] . Chen I.J., Chou C.C., Liu C.L., 2014

机译：Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein

Crystallization and preliminary crystallographic studies on the chromo shadow domain (CSD) of mouse heterochromatin protein M31.

摘要

著录项

相似文献

相关主题

期刊订阅