Purification, crystallization and preliminary X-ray diffraction results of human 17beta-hydroxysteroid dehydrogenase type 5.

Zhou M; Qiu W; Chang HJGangloff ALin SX

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, crystallization and preliminary X-ray diffraction results of human 17beta-hydroxysteroid dehydrogenase type 5.

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Purification, crystallization and preliminary X-ray diffraction results of human 17beta-hydroxysteroid dehydrogenase type 5.

机译：净化、结晶和初步的x射线衍射结果的人类17 beta-hydroxysteroid脱氢酶类型5。

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17beta-Hydroxysteroid dehydrogenases (17beta-HSDs) catalyze the last step in the biosynthesis of all androgens and estrogens, thus playing a pivotal role in sex-hormone metabolism. Human 17beta-HSD type 5 (17beta-HSD5) catalyzes hydride transfer at the 17beta-hydroxy position, but possesses high sequence homology to 3alpha-hydroxysteroid dehydrogenases (3alpha-HSD). Two crystal forms of 17beta-HSD5 in an enzyme-testosterone-NADP ternary complex have been obtained under different crystallization conditions. A form I crystal obtained at pH 8.5 diffracted to 1.32 A. It belonged to space group P2(1), with unit-cell parameters a = 47.41, b = 77.16, c = 48.67 A, beta = 116.32 degrees. Form II crystals obtained at pH 6.5 diffracted to 2.0 A and belonged to space group P6(3), with unit-cell parameters a = b = 110.58, c = 56.89 A.

机译：17 beta-hydroxysteroid脱氢酶(beta-hsds 17日)催化生物合成的最后一步雄激素和雌激素,从而发挥至关重要在性激素代谢中的作用。5类型(17 beta-hsd5)催化氢化转移在17 beta-hydroxy位置,但拥有高3 alpha-hydroxysteroid序列同源性脱氢酶(3 alpha-hsd)。17 beta-hsd5 enzyme-testosterone-NADP取得了三元复杂不同的结晶条件。获得的晶体在pH值1.32 8.5衍射。它属于空间群P2(1),与单胞参数= 47.41,= 77.16 b, c = 48.67,β= 116.32度。在pH值2.0 6.5衍射和属于空间群P6(3),晶胞参数=b = 110.58, c = 56.89。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|1048-1050|共3页
作者
Zhou M; Qiu W; Chang HJGangloff ALin SX;
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Molecular Endocrinology Laboratory, CHUL Research Center, Laval University, Canada.;

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正文语种英语
中图分类化学;
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Oxidoreductases; cell parameter; X-ray diffractioncrystals17 beta-Hydroxysteroid DehydrogenasesHydride transferspace groupsCrystallization17-Hydroxysteroid DehydrogenasesHomologous SequencesDehydrogenasesAndrogensEstrogens;

机译：氧化还原酶,细胞参数;x射线diffractioncrystals17 beta-HydroxysteroidDehydrogenasesHydride transferspacegroupsCrystallization17-HydroxysteroidDehydrogenasesHomologous;

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Purification, crystallization and preliminary X-ray diffraction results of human 17beta-hydroxysteroid dehydrogenase type 5.

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