Purification, crystallization and molecular symmetry of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis.

Vogan EM; Bellamacina CR; He XFoxman BMRinge DLiu HWPetsko GA

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, crystallization and molecular symmetry of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis.

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Purification, crystallization and molecular symmetry of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis.

机译：净化、结晶和分子对称CDP-D-glucose 4日6-dehydratase鼠疫的伪。

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摘要

The enzyme CDP-D-glucose 4,6-dehydratase (EC 4.2.1.45) is an NAD(+)-dependent oxidoreductase which catalyzes the irreversible conversion of CDP-D-glucose to CDP-4-keto-6-deoxy-D-glucose. The product of this reaction is an intermediate in the synthesis of all CDP-linked 3,6-dideoxyhexoses, an important class of antigenic determinants found in the lipopolysaccharide layer of Gram-negative bacteria. Crystals of a recombinant form of this enzyme from Yersinia pseudotuberculosis have been grown in two crystal forms, both possessing pseudo-translational non-crystallographic symmetry, with dramatically different diffraction characteristics. A complete 1.8 A data set has been collected from the primitive orthorhombic crystal form, for which the non-crystallographic symmetry is described in detail.

机译：这种酶CDP-D-glucose 4, 6-dehydratase (EC4.2.1.45)是一种NAD(+)端依赖氧化还原酶催化的不可逆转换CDP-D-glucose CDP-4-keto-6-deoxy-D-glucose。这个反应是一个中间的产物在所有CDP-linked的合成3, 6-dideoxyhexoses,一个重要的一类抗原的决定因素脂多糖层革兰氏阴性细菌。酶从鼠疫的伪生长在两种晶体形式,都拥有pseudo-translational non-crystallographic对称,截然不同的衍射特征。从原始的斜方晶系的收集晶体形式,non-crystallographic对称是详细描述。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|370-373|共4页
作者
Vogan EM; Bellamacina CR; He XFoxman BMRinge DLiu HWPetsko GA;
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作者单位

Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA. vogan@crystal.harvard.edu;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Yersinia pseudotuberculosis; Antigenic determinants; SymmetryHydro-Lyasescrystal outline;

机译：决定因素;SymmetryHydro-Lyasescrystal大纲;

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Purification, crystallization and molecular symmetry of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis.

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