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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray crystallographic studies on the parD-encoded protein Kid from Escherichia coli plasmid R1.
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Crystallization and preliminary X-ray crystallographic studies on the parD-encoded protein Kid from Escherichia coli plasmid R1.

机译：结晶和初步的x射线parD-encoded晶体研究蛋白质从大肠杆菌质粒R1的孩子。

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摘要

DNA replication in Escherichia coli and therefore bacterial proliferation relies upon the efficient functioning of the DnaB helicase. The toxin protein Kid from the plasmid-stability system parD encoded on plasmid R1 of E. coli is thought to target and block DnaB-dependent DNA replication. The toxicity of Kid is antagonized through interaction with the Kis antidote protein and the resultant complex can then act as a transcriptional regulator for the parD system. Crystals of selenomethionine-incorporated Kid have been obtained by the hanging-drop vapour-diffusion method using potassium phosphate as the precipitant. The crystals belong to the monoclinic system, space group P2(1), have unit-cell parameters a = 32.9, b = 45.0, c = 64.4 A, beta = 96.2 degrees and diffract to a d(min) of better than 1.8 A on a synchrotron-radiation source. The determination of the structure of Kid will permit a better understanding of its interactions with DnaB and Kis and allow the evolutionary relationships of Kid to othertoxins of plasmid and chromosomal origin to be explored.
机译：在大肠杆菌,因此DNA复制细菌增殖依赖于有效功能的DnaB解旋酶。蛋白质的孩子从plasmid-stability系统豹在大肠杆菌的质粒R1编码思想目标和块DnaB-dependent DNA复制。通过与克义斯解药蛋白质的交互和可以作为合成复杂转录监管机构伙伴系统。晶体的selenomethionine-incorporated孩子获得了悬滴使用磷酸钾vapour-diffusion方法作为沉淀剂。单斜晶系,空间群P2 (1)晶胞参数= 32.9,= 45.0 b, c = 64.4,β= 96.2度和衍射d(分钟)比1.8的电脑同步源。将允许更好的理解它与DnaB交互和ki和允许孩子othertoxins的进化关系质粒和染色体起源的探索。

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