Crystallization and preliminary X-ray studies of the single-stranded DNA-binding protein from Mycobacterium tuberculosis.

Saikrishnan K; Jeyakanthan J; Venkatesh JAcharya NPurnapatre KSekar KVarshney UVijayan M

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Crystallization and preliminary X-ray studies of the single-stranded DNA-binding protein from Mycobacterium tuberculosis.

机译：结晶和初步的x射线的研究单链dna结合蛋白结核分枝杆菌。

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Single-stranded DNA-binding proteins play an important role in DNA replication, repair and recombination. The protein from Mycobacterium tuberculosis (MtSSB) is a tetramer with 164 amino-acid residues in each subunit. The protein readily crystallizes in space group P3(1)21 (or P3(2)21) at pH 7.4 under appropriate conditions. Under different conditions, but at the same pH, orthorhombic crystals belonging to space group I222 or I2(1)2(1)2(1) were obtained after several months. Similar orthorhombic crystals were obtained when protein samples stored for several months were used for crystallization. The orthorhombic crystals obtained in different experiments, though similar to one another, exhibited variations in unit-cell parameters, presumably on account of different extents of proteolytic cleavage of the C-terminal region. Molecular-replacement calculations using different search models did not yield the structure. As part of attempts to solve the structure using isomorphous replacement, a good mercury derivative of the trigonal crystal has been prepared.

机译：单链dna结合蛋白质发挥重要的角色在DNA复制,修复和重组。与164年结核病(MtSSB)是一个四聚物每个亚基氨基酸残基。容易结晶的空间群P3(1) 21(或P3(2) 21)在适当的条件下在pH值为7.4。在不同条件下,但在相同的pH值,斜方晶系的晶体属于空间群I222或I2(1) 2(1) 2(1)几个后得到个月。当蛋白质样品存储数个月用于结晶。斜方晶系的晶体在不同实验中,虽然彼此相似,表现出晶胞参数的变化大概的不同区段的蛋白水解c端地区的乳沟。Molecular-replacement计算使用不同的搜索模型没有产生结构。使用同形结构替代,一个好的水星三方晶系的晶体的导数被准备。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|327-329|共3页
作者
Saikrishnan K; Jeyakanthan J; Venkatesh JAcharya NPurnapatre KSekar KVarshney UVijayan M;
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作者单位

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.;

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正文语种英语
中图分类化学;
关键词
Single-Stranded DNA Binding Proteins; Mycobacterium tuberculosis; crystalsDNA-Binding Proteinsmercury derivativeTetramersX-raysCrystallizationorthorhombic system;

机译：单链DNA结合蛋白;分枝杆菌肺结核;crystalsDNA-Binding Proteinsmercury;

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Crystallization and preliminary X-ray studies of the single-stranded DNA-binding protein from Mycobacterium tuberculosis.

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