Crystallization of PNMT, the adrenaline-synthesizing enzyme, is critically dependent on a high protein concentration.

Begun J; McLeish MJ; Caine JMPalant EGrunewald GLMartin JL

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of PNMT, the adrenaline-synthesizing enzyme, is critically dependent on a high protein concentration.

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Crystallization of PNMT, the adrenaline-synthesizing enzyme, is critically dependent on a high protein concentration.

机译：PNMT的结晶adrenaline-synthesizing酶,是批判性的依赖于高蛋白质浓度。

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Phenylethanolamine N-methyltransferase, PNMT, utilizes the methylating cofactor S-adenosyl-L-methionine to catalyse the synthesis of adrenaline. Human PNMT has been crystallized in complex with an inhibitor and the cofactor product S-adenosyl-L-homocysteine using the hanging-drop technique with PEG 6000 and lithium chloride as precipitant. A critical requirement for crystallization was a high enzyme concentration (>90 mg ml(-1)) and cryocrystallography was used for high-quality data measurement. Diffraction data measured from a cryocooled crystal extend to a resolution of 2.3 A. Cryocooled crystals belong to space group P4(3)2(1)2 and have unit-cell parameters a = b = 94.3, c = 187.7 A.

机译：Phenylethanolamine N-methyltransferase PNMT,利用甲基化代数余子式S-adenosyl-L-methionine催化合成肾上腺素。在复杂的抑制剂和辅因子产品S-adenosyl-L-homocysteine使用悬滴法与PEG 6000和锂氯作为沉淀剂。结晶是一个高酶浓度(> 90毫克毫升(1))cryocrystallography用于高质量数据测量。cryocooled晶体扩展的决议2.3。P4(3) 2(1) 2,晶胞参数a = b =94.3, c = 187.7。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|314-315|共2页
作者
Begun J; McLeish MJ; Caine JMPalant EGrunewald GLMartin JL;
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作者单位

Centre for Drug Design and Development and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia.;

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正文语种英语
中图分类化学;
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lithium chloride; protein concentration; Phenylethanolamine N-MethyltransferaseCrystallization;

机译：氯化锂;蛋白质浓度;PhenylethanolamineN-MethyltransferaseCrystallization;

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Crystallization of PNMT, the adrenaline-synthesizing enzyme, is critically dependent on a high protein concentration.

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