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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase.
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Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase.

机译：季戊四醇propoxylate:一个新的结晶代理和冷冻保护剂诱导晶体生长的2-methylcitrate脱水酶。

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摘要

In the search for macromolecular crystallization conditions, the precipitant is probably the most important variable, such that when problematic crystals are encountered there is always the question of whether an alternative precipitant might resolve the problem. During an effort to obtain high-quality crystals of several problematic proteins, two new agents, pentaerythritol propoxylate and pentaerythritol ethoxylate, yielded well ordered quality crystals where more traditional precipitants were unsuccessful. Pentaerythritol propoxylate and pentaerythritol ethoxylate contain a pentaerythritol backbone to which organic polymers are bound, forming a branched polymer. As such, they are larger than small organic precipitants such as low molecular-weight alcohols or 2-methyl-2,4-pentanediol, but behave differently to polyethylene glycols. These compounds have been used to crystallize an enzyme encoded by the Salmonella enterica prpD gene that catalyzes the dehydration of 2-methylcitrate to form 2-methyl-cis-aconitate. While the PrpD protein has crystallized readily under a number of conditions, the resultant crystals were unsuitable for a crystal structure determination. The new crystals obtained with 25-40% pentaerythritol propoxylate belong to the orthorhombic space group C222(1), with unit-cell parameters a = 73.2, b = 216.4, c = 214.3 A, and diffract beyond 2.0 A with synchrotron radiation. A further benefit of this precipitant for crystallization is its ability to function as a cryoprotectant, allowing the crystals to be transferred directly from the mother liquor to the nitrogen stream at 113 K.
机译：在寻找大分子结晶条件下,沉淀剂可能是最重要的变量,这样当问题水晶是总有遇到是否另一种沉淀剂可能会解决问题。获得高质量的晶体的几个有问题的蛋白质,两个新的代理,季戊四醇propoxylate和季戊四醇乙氧基化物,产生有序晶体质量更传统的沉淀剂在哪里成功。季戊四醇乙氧基化物包含一个季戊四醇有机骨干聚合物绑定,形成支化聚合物。因此,他们是比小型有机食品低分子量等沉淀剂醇或2-methyl-2 4-pentanediol,但行为不同的聚乙二醇。化合物被用来结晶的一种酶沙门氏菌血清prpD基因编码催化2-methylcitrate的脱水2-methyl-cis-aconitate形式。蛋白质结晶很容易在一个数字的条件下,合成水晶不适合一个晶体结构的决心。新获得的晶体与25 - 40%季戊四醇propoxylate属于斜方晶系的空间群C222(1),与单胞参数= 73.2,= 216.4 b, c = 214.3,用同步辐射衍射超过2.0。进一步的好处这突如其来的结晶是其作为的能力冷冻保护剂,使晶体将直接从母液氮流在113 K。

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