Purification and crystallization of the yeast elongation factor eEF2.

Jorgensen R; Carr Schmid A; Ortiz PAKinzy TGAndersen GR

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Purification and crystallization of the yeast elongation factor eEF2.

机译：纯化和结晶的酵母eEF2伸长因素。

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Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

机译：酿酒酵母的晶体延长因子2 (eEF2)在复杂的国内生产总值得到vapour-diffusion技术从工业快速净化后酵母。晶体衍射,属于2.85空间群P2(1) 2(1) 2(1)。表达功能histidine-tagged eEF2蛋白质进一步允许的唯一形式促进净化的因素结构和功能的研究。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第4期|712-715|共4页
作者
Jorgensen R; Carr Schmid A; Ortiz PAKinzy TGAndersen GR;
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作者单位

Institute of Molecular and Structural Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus, Denmark.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Yeast; Peptide Elongation Factor 2; PurificationSaccharomyces cerevisiaecrystalsPeptide Elongation FactorsCrystallization;

机译：酵母;肽伸长的因素2, PurificationSaccharomycescerevisiaecrystalsPeptide伸长FactorsCrystallization;

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Purification and crystallization of the yeast elongation factor eEF2.

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