...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima.
【24h】

Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima.

机译：结晶和初步的x射线描述的耐热性的果胶酸裂解酶从Thermotoga maritima。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pectate lyase is an enzyme involved in the degradation of the pectate portion of the primary plant cell wall. A recombinant pectate lyase from Thermotoga maritima where three of the four cysteine residues have been mutated (C132I, C156N, C194L) has been crystallized. Crystals of the same morphology and trigonal space group R3 with similar unit-cell parameters were obtained under two different conditions. The first, 0.3 M (NH(4))H(2)PO(4) pH 4.2, gave crystals with a maximum size of 0.4 x 0.2 x 0.2 mm in one week that diffracted to a resolution of 1.87 A and had unit-cell parameters a = b = 80.6, c = 148.8 A. The second, 0.1 M sodium acetate, 6%(w/v) PEG 4000 pH 6.5, gave the same size crystals in two weeks that diffracted to a resolution of 2.1 A and had unit-cell parameters a = b = 80.0, c = 150.1 A.
机译：果胶酸裂解酶是一种酶参与降解果胶酸盐的主要的一部分植物细胞壁。Thermotoga maritima三四个半胱氨酸残基突变(C132I,C156N C194L)结晶。相同的形态和三角形空间群R3也获得了类似的晶胞参数在两种不同条件下。(NH (4) H (2) PO (4) pH值4.2,给晶体了最大尺寸0.4 x 0.2 x 0.2毫米的一个星期衍射分辨率为1.87和晶胞参数a = b = 80.6, c = 148.8。第二,0.1醋酸钠,6% (w / v)挂钩4000年pH值6.5,给了在两个同样大小的晶体周,衍射分辨率为2.1,晶胞参数a = b = 80.0, c =150.1。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号