Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.

Pereira de Jesus K; Serre L; Hervouet NBouckson Castaing VZelwer CCastaing B

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.

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Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.

机译：结晶和初步的x射线晶体之间的一种复杂的研究Lactococcus lactis台塑dna修复酶和一个基本的网站包含DNA。

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For protein-DNA complex crystallization, the choice of the DNA fragment is crucial. With the aim of crystallizing the 31 kDa Fpg DNA-repair enzyme bound to DNA, oligonucleotide duplexes varying in length, sequence, end type and nature of the specific DNA target site were used. Crystals of several protein-DNA combinations grew from solutions containing both polyethylene glycol and salt. This systematic crystallization screening followed by optimization of the crystallization conditions by microseeding led to crystals of Fpg bound to a 13 base-pair duplex DNA carrying the 1,3-propanediol abasic site analogue which are suitable for crystallographic analysis. Complete native data sets have been collected to 2.1 A resolution.

机译：对于protein-DNA复杂的结晶,DNA片段的选择是至关重要的。目标31台塑dna修复kDa的结晶酶与DNA寡核苷酸工器不同长度、序列类型和性质目标站点的特定DNA。晶体的几个protein-DNA组合了从解决方案包含聚乙烯乙二醇和盐。筛选优化的紧随其后由microseeding导致结晶条件台塑绑定到一个水晶的13个碱基对双工1,3 -丙二醇形成规模化DNA携带基本的网站适用于晶体的模拟分析。收集到2.1一项决议。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第4期|679-682|共4页
作者
Pereira de Jesus K; Serre L; Hervouet NBouckson Castaing VZelwer CCastaing B;
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作者单位

Centre de Biophysique Moleculaire UPR4301, affiliated to the University of Orleans, CNRS, Rue Charles Sadron, 45071 Orleans CEDEX 02, France.;

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正文语种英语
中图分类化学;
关键词
N-glycoside hydrolases; DNA; N-Glycosyl HydrolasesDNA fragmentcrystalsstreptococcus lactisLactic streptococcirepair enzymeCrystallization;

机译：N-glycoside水解酶;基因;N-GlycosyllactisLactic streptococcirepairenzymeCrystallization;

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Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.

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