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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of the functional domain of human thrombopoietin using an antigen-binding fragment derived from neutralizing monoclonal antibody.
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Crystallization of the functional domain of human thrombopoietin using an antigen-binding fragment derived from neutralizing monoclonal antibody.

机译：结晶的功能域的人类促血小板生成素使用antigen-binding片段来自中和单克隆抗体。

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摘要

Thrombopoietin (TPO) is a cytokine which primarily stimulates megakaryocytopoiesis and thrombopoiesis. The functional domain of TPO (TPO(163)) consisting of the N-terminal 163 amino acids was prepared and crystallized. Since the crystallization of TPO(163) was unsuccessful using the standard screening methods, a Fab fragment derived from a neutralizing monoclonal antibody was used for crystallization. It was found that the TPO(163)-Fab complex crystallized reproducibly in 0.1 M potassium phosphate buffer pH 6.0 containing 20-25% polyethylene glycol 4000. Thin crystals (0.2 x 0.2 x 0.02 mm) grew in two space groups: P2(1), with unit-cell parameters a = 133.20, b = 46.71, c = 191.47 A, beta = 90.24 degrees, and C2, with unit-cell parameters a = 131.71, b = 46.48, c = 184.63 A, beta = 90.42 degrees. The results of a molecular-replacement analysis indicate that the Fab molecules interact with each other and provide a suitable interface for crystallization.
机译：促血小板生成素(TPO)是一个主要的细胞因子刺激megakaryocytopoiesis,血栓形成。(传真照片(163))组成的氨基端163个氨基酸酸制备和结晶。房产申诉专员署(163)的结晶是不成功的使用标准的筛选方法,一个工厂片段来自中和单克隆抗体用于结晶。发现TPO(163)工厂复杂的结晶地在0.1磷酸钾缓冲pH值6.0包含20 - 25%的聚乙二醇4000. 两个空间组:P2(1),晶胞参数= 133.20,= 46.71 b, c = 191.47,β= 90.24度,和C2,晶胞参数= 131.71,= 46.48 b, c = 184.63,β= 90.42度。molecular-replacement分析表明工厂分子相互作用提供一个合适的界面结晶。

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