Thaumatin crystallization aboard the International Space Station using liquid-liquid diffusion in the Enhanced Gaseous Nitrogen Dewar (EGN).

Barnes CL; Snell EH; Kundrot CE

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Thaumatin crystallization aboard the International Space Station using liquid-liquid diffusion in the Enhanced Gaseous Nitrogen Dewar (EGN).

机译：奇异果甜蛋白结晶国际上空间站使用液-液扩散增强的气态氮杜瓦(EGN)。

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This paper reports results from the first biological crystal-growth experiment on the International Space Station (ISS). Crystals of thaumatin were grown using liquid-liquid diffusion in Tygon tubing transported in the Enhanced Gaseous Nitrogen Dewar (EGN). Different volume ratios and concentrations of protein and precipitant were used to test different adaptations of the vapor-diffusion crystallization recipe to the liquid-liquid diffusion method. The EGN warmed up from 77 to 273 K in about 4 d, about the same time it took to warm from 273 to 293 K. The temperature within the EGN was 293-297 K for the majority of the experiment. Air gaps that blocked liquid-liquid diffusion formed in the tubes. Nonetheless, crystals were grown. Synchrotron diffraction data collected from the best space-grown crystal extended to 1.28 A, comparable to previous studies of space-grown thaumatin crystals. The resolution of the best ground-control crystal was only 1.47 A. It is not clear if the difference in diffraction limit arises from factors other than crystal size. Improvements in temperature control and the elimination of air gaps are needed, but the results show that the EGN on the ISS can be used to produce space-grown crystals that diffract to high resolution.

机译：本文从第一个报告结果生物晶体生长实验国际空间站(ISS)。奇异果甜蛋白是利用液-液扩散在聚乙烯管材运输增强气态氮杜瓦(EGN)。的蛋白质和体积比率和浓度沉淀剂被用来测试不同适应的扩散结晶液-液配方扩散的方法。在4 d 273 K,大约在同一时间从273到293 K温暖。EGN是293 - 297 K的多数实验。扩散管中形成。晶体生长。从最好的收集空间长大晶体扩展到1.28,与之前的研究空间长大奇异果甜蛋白晶体。分辨率最好的疗法在水晶只有1.47。衍射极限起源于以外的因素晶体的大小。和消除空气差距是必要的,但是结果表明,EGN在国际空间站用于生产空间长大晶体高分辨率的衍射。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第5期|751-760|共10页
作者
Barnes CL; Snell EH; Kundrot CE;
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作者单位

Mail Code SD46, Marshall Space Flight Center, AL 35812, USA.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
thaumatin protein, plant; AIR GAP; DewarsPlant ProteinsSWEETENING AGENT(CODEX)crystalsGasesSpace FlightCrystallization;

机译：奇异果甜蛋白的蛋白质,植物,气隙;DewarsPlant(法典)crystalsGasesSpace ProteinsSWEETENING代理FlightCrystallization;

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Thaumatin crystallization aboard the International Space Station using liquid-liquid diffusion in the Enhanced Gaseous Nitrogen Dewar (EGN).

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