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首页> 外文期刊>International Journal of Environmental Impacts: Management, Mitigation and Recovery >IMPACT OF GASEOUS NO_2 ON P. FLUORESCENS STRAIN IN THE MEMBRANE ADAPTATION AND VIRULENCE
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IMPACT OF GASEOUS NO_2 ON P. FLUORESCENS STRAIN IN THE MEMBRANE ADAPTATION AND VIRULENCE

机译：NO_2气体在p .荧光应变的影响适应和细胞膜毒性

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摘要

Nowadays air pollution is increasing due to anthropogenic activity. Among all air pollutants, nitrogen oxides (NOx) such as NO_2 are predominant. It is well known that those compounds exhibit direct toxic effects on human health. However, microorganisms are also exposed to them, but the effect of NOx on the virulence of air microbiota is still poorly understood. In this study, we evaluated the impact of NO_2 on the adaptability and virulence of an airborne strain of P. fluorescens, MFA76a, by exposition of this strain to 45 ppm of NO_2. The growth kinetics and cultivability were analysed. A decrease of cultivability coupled with an increase of the lag phase was observed suggesting a potential toxicity of NO_2. Since NOx particularly target lipids, the membrane permeability was assessed thanks to Live Dead tests and confocal microscopy. A significant alteration of membrane permeability was observed. Furthermore, more abundant bacterial aggregates were detected compared to the control. Thus, a lipidomic study was performed using MALDI-TOF MS Imaging coupled to HPTLC. Interestingly, bacteria exposed to NO_2 were lacking one putative glycerophospholipid molecule. In agreement with a previous study from Kondakova et al., these data demonstrate the adaptation potential of P. fluorescens MFAF76a to an air pollutant such as NO_2.
机译：如今空气污染是由于增加人为活动。氮氧化物(NOx) NO_2等占主导地位的。化合物表现出直接毒性对人类的影响健康。对他们来说,但氮氧化物的毒性的影响空气微生物群仍知之甚少。这项研究中,我们评估NO_2的影响一个机载的适应性和毒性应变的荧光,MFA76a博览会这株45 NO_2的ppm。动力学和cultivability进行分析。减少cultivability加上一个增长停滞阶段的建议NO_2的潜在毒性。特别是目标脂质膜磁导率是评估由于生活死了测试和共焦显微镜。观察膜通透性的改变。此外,更丰富的细菌总量检测与控制。使用女士MALDI-TOF lipidomic研究执行成像的效果。暴露于NO_2缺乏一个公认的glycerophospholipid分子。之前的研究从Kondakova et al .,这些数据适应展示潜力的P。荧光MFAF76a空气污染物等NO_2。

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