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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preliminary crystallographic analysis of the cysteine desulfurase IscS from Escherichia coli.
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Preliminary crystallographic analysis of the cysteine desulfurase IscS from Escherichia coli.

机译：初步的晶体的分析半胱氨酸desulfurase isc从大肠杆菌。

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摘要

IscS is a widely distributed cysteine desulfurase that catalyzes the pyridoxal phosphate dependent beta-elimination of sulfur from L-cysteine and plays a central role in the delivery of sulfur to a variety of metabolic pathways. Crystals of Escherichia coli IscS have been obtained by the hanging-drop vapor-diffusion method using polyethylene glycol (PEG) as a precipitant. Initial seed crystals were obtained using PEG 6000 and sodium acetate, and diffraction-quality crystals were grown using a mixture of PEG 2000 and PEG 10 000 in the presence of sodium citrate. A complete native X-ray diffraction data set was collected from a single crystal at 103 K to a resolution of 2.1 A. The crystals belong to space group P2(1)2(1)2(1) and have unit-cell parameters a = 73.7086, b = 101.9741, c = 108.617 A (alpha = beta = gamma = 90 degrees ). Analysis of the Matthews equation and self-rotation function suggest two molecules per asymmetric unit, consistent with the presence of a single dimeric molecule.
机译：isc半胱氨酸desulfurase的分布很广泛催化磷酸吡哆醛的依赖从半胱氨酸和beta-elimination硫在交付硫过程中扮演着重要的角色不同的代谢途径。大肠杆菌isc已经获得的悬滴扩散方法使用聚乙二醇(PEG)作为沉淀剂。初始种子晶体得到使用挂钩6000年,醋酸钠,diffraction-quality晶体生长使用挂钩2000的混合物和挂钩10 000年柠檬酸钠的存在。一个完整的本地x射线衍射数据集收集从单晶在103 K2.1解决。集团P2(1) 2(1) 2(1),晶胞参数b = 101.9741 = 73.7086, c = 108.617(α=β=γ= 90度)。马修斯方程和固有转动功能建议每两个分子不对称单位,一致的存在一个二聚的分子。

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