Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature.

Thiyagarajan S; Satheesh Kumar P; Rajan SSGautham N

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Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature.

机译：结构在293 K d (TGCGCA) 2:比较的序列和温度的影响。

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The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.

机译：的晶体结构hexameric DNA片段与序列d (TGCGCA)(2)已经解决和细化1.64 293 K的决议。分子实行左撇子z形螺旋构象,在交流中很常见pyrimidine-purine序列。碱基对的两个终端不扰乱很大程度上的结构。几个sequence-specific显微结构的变化是明显的。在120 K序列确定有所涉及不同的结晶条件先前报道[哈珀et al。(1998),学报结晶的。现在的结构,在较低的温度和与d (CGCGCG)(2)显示序列的变化大于的效果温度和变化的综合效应环境。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第8期|1381-1384|共4页
作者
Thiyagarajan S; Satheesh Kumar P; Rajan SSGautham N;
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作者单位

Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India.;

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正文语种英语
中图分类化学;
关键词
TPD52L2 gene; crystal structure; DNA fragmentTemperatureOligodeoxyribonucleotides;

机译：TPD52L2基因;晶体结构;DNA;

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Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature.

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