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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction studies of the transcriptional regulator TraR bound to its cofactor and to a specific DNA sequence.
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Crystallization and preliminary X-ray diffraction studies of the transcriptional regulator TraR bound to its cofactor and to a specific DNA sequence.

机译：结晶和初步的x射线衍射研究转录监管机构TraR绑定到它的代数余子式和到一个特定的DNA序列。

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摘要

TraR is an Agrobacterium tumefaciens transcriptional regulator which binds the pheromone N-3-oxooctanoyl-L-homoserine lactone (AAI) in response to the bacterial population density. The TraR-AAI complex dimerizes and interacts with a specific 18-base-pair DNA sequence (TraBox), activating promoters containing this site. TraR was overexpressed and purified from Escherichia coli. Crystals of the ternary complex, in which dimeric TraR-AAI is bound to the TraBox sequence, have been obtained by the vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.99, b = 94.67, c = 209.66 A, with two (TraR-AAI)(2)-TraBox complexes in the asymmetric unit. A three-wavelength MAD data set for the seleno-L-methionine-substituted form has been collected to a resolution of 3 A. 20 of the 24 crystallographically independent selenium sites were located as part of the MAD-phasing process.
机译：TraR根癌土壤杆菌转录监管机构的结合信息素N-3-oxooctanoyl-L-homoserine内酯(AAI)的细菌种群密度。与一个特定的18-base-pair DNA序列(TraBox),激活启动子包含了这个网站。纯化大肠杆菌。三元复杂,二聚的TraR-AAI绑定到TraBox序列,取得了由vapour-diffusion方法。属于空间群P2 (1) 2 (1) 2 (1)晶胞参数= 66.99,b = 94.67, c =209.66,有两个(TraR-AAI) (2) -TraBox复合物在不对称单位。seleno-L-methionine-substituted数据集形式收集3的一项决议。20 24结晶学的独立硒网站的一部分MAD-phasing过程。

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