Crystallization and preliminary X-ray diffraction analysis of the trigonal crystal form of Saccharomyces cerevisiae alcohol dehydrogenase I: evidence for the existence of Zn ions in the crystal.

Kim KJ; Howard AJ

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of the trigonal crystal form of Saccharomyces cerevisiae alcohol dehydrogenase I: evidence for the existence of Zn ions in the crystal.

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Crystallization and preliminary X-ray diffraction analysis of the trigonal crystal form of Saccharomyces cerevisiae alcohol dehydrogenase I: evidence for the existence of Zn ions in the crystal.

机译：结晶和初步的x射线衍射三方晶系的晶体形式的分析酿酒酵母乙醇脱氢酶我:锌离子的存在的证据晶体。

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Saccharomyces cerevisiae alcohol dehydrogenase I crystallized as trigonal plates using 20% 2-propanol and 20% PEG 4000 in 0.1 M sodium citrate buffer pH 5.6 in the presence of 1 mM NAD(+). The crystals diffract to 3.0 A resolution and belong to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 146.3, c = 68.1 A, alpha = beta = 90, gamma = 120 degrees. X-ray data were collected from frozen crystals at the 17-ID beamline of the Advanced Photon Source. A Zn fluorescence scan of the crystal produced a peak at 9671.6 eV, suggesting the existence of Zn ions in the crystal.

机译：酿酒酵母乙醇脱氢酶使用20%结晶为三方晶系的盘子4000年丙胺和20% PEG 0.1钠柠檬酸缓冲液pH值5.6的1毫米NAD(+)。和属于三方晶系的空间群P3(1) 21或P3(2) 21日晶胞参数a = b = 146.3,c = 68.1,α=β= 90,γ= 120度。晶体在17-ID beamline先进光子源。晶体产生峰值为9671.6 eV,暗示锌离子在晶体的存在。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第8期|1332-1334|共3页
作者
Kim KJ; Howard AJ;
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作者单位

Industrial Macromolecular Crystallography Association Access Team (IMCA-CAT), Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.;

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正文语种英语
中图分类化学;
关键词
zinc; Alcohol Dehydrogenase I; Saccharomyces cerevisiaecrystalscrystal outlineX-ray diffraction analysisAlcohol DehydrogenaseCrystallization;

机译：锌;乙醇脱氢酶;酿酒cerevisiaecrystalscrystal outlineX-ray衍射analysisAlcoholDehydrogenaseCrystallization;

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Crystallization and preliminary X-ray diffraction analysis of the trigonal crystal form of Saccharomyces cerevisiae alcohol dehydrogenase I: evidence for the existence of Zn ions in the crystal.

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