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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray data of a bifunctional peroxiredoxin from poplar.
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Crystallization and preliminary X-ray data of a bifunctional peroxiredoxin from poplar.

机译：结晶和初步的x射线数据从杨树双官能的酶类。

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摘要

Two variants (wild type and V152C mutant) of a bifunctional poplar peroxiredoxin have been overexpressed in Escherichia coli cells. The two recombinant enzymes were purified and crystallized using the hanging-drop vapour-diffusion technique. Data sets were collected to 1.62 and 2.48 A resolution using X-ray synchrotron-source radiation from two crystal forms of wild-type peroxiredoxin which belonged to the monoclinic space group P2(1) (with unit-cell parameters a = 59.26, b = 68.80, c = 75.71 A, beta = 93.45 degrees ) and to the orthorhombic space group P2(1)2(1)2 (with unit-cell parameters a = 64.70, b = 130.73, c = 35.59 A), respectively. Data were also collected to 2.17 A resolution using a home X-ray source from a V152C peroxiredoxin crystal which belongs to the triclinic space group (P1), with unit-cell parameters a = 36.65, b = 41.53, c = 58.06 A, alpha = 70.52, beta = 93.45, gamma = 64.31 degrees. Phases have been obtained using molecular replacement with the structure of human peroxiredoxin V (PDB code 1hd2) as a search model. Refinement of the structures is in progress.
机译：两个变量(野生型和V152C突变)双官能杨树的酶类在大肠杆菌细胞中过表达。重组酶纯化和使用悬滴结晶vapour-diffusion技术。收集使用1.62和2.48一项决议x射线synchrotron-source辐射2晶体形式的野生型的酶类属于单斜晶体的空间群P2 (1)(晶胞参数= 59.26,b = 68.80,c = 75.71,β= 93.45度)和斜方晶系的空间群P2 (1) 2 (1) 2 (晶胞参数= 64.70,b = 130.73, c =分别为35.59)。2.17解决方案使用x射线源从V152C酶类水晶属三斜晶系的空间组织(P1),晶胞参数= 36.65,= 41.53 b, c = 58.06,α= 70.52,β= 93.45,γ= 64.31度。分子替代人类的结构酶类V (PDB代码1 hd2)作为搜索模型。的进步。

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