Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site.

Lariviere L; Kurzeck J; Aschke Sonnenborn URuger WMorera S

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Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site.

机译：结晶和初步的晶体研究三元的T4噬菌体之间的复杂beta-glucosyltransferase,尿苷diphosphoglucose包含一个和一个DNA片段基本的网站。

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A base-flipping phenomenon has been established for DNA methyltransferases and for DNA base-excision repair glycosylases and is likely to prove general for enzymes that need access to DNA bases to undergo chemical reaction. T4 phage beta-glucosyltransferase (BGT) is a good candidate for this novel mechanism. In order to confirm this, BGT was crystallized with an abasic site-containing DNA and uridine diphosphoglucose (UDP-glucose). The crystallization strategy is described. A complete data set was collected at 1.8 A resolution on a Cu Kalpha rotating-anode X-ray source. Molecular replacement was performed and the initial electron-density maps clearly show bound DNA.

机译：已经建立了一个base-flipping现象为DNA甲基转移酶和DNAbase-excision修复糖基化酶和可能证明一般需要访问的酶DNA碱基进行化学反应。beta-glucosyltransferase(英国达人)是一个很好的这部小说候选人机制。证实了这一点,踏进结晶步行不能的site-containing DNA和尿苷diphosphoglucose(UDP-glucose)。描述。1.8决议铜Kalpha旋转阳极x射线源。和初始电子密度地图清楚显示绑定DNA。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第9期|1484-1486|共3页
作者
Lariviere L; Kurzeck J; Aschke Sonnenborn URuger WMorera S;
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作者单位

Laboratoire d'Enzymologie et Biochimie Structurales, UPR 9063 CNRS, Batiment 34, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Uridine Diphosphate Glucose; DNA; DNA fragmentBase Excision RepairGlucosyltransferasesBacteriophage T4Crystallization;

机译：切除RepairGlucosyltransferasesBacteriophage;

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Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site.

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