Crystallization and preliminary crystallographic analysis of a D-aminoacylase from Alcaligenes faecalis DA1.

Hsu CS; Chen SJ; Tsai YCLin TWLiaw SHWang AH

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Crystallization and preliminary crystallographic analysis of a D-aminoacylase from Alcaligenes faecalis DA1.

机译：结晶和初步的晶体分析D-aminoacylase产碱杆菌属

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D-Aminoacylases catalyze the hydrolysis of N-acyl-D-amino acids into D-amino acids with the aid of zinc ions. The first D-aminoacylase crystal from Alcaligenes faecalis has been obtained in hanging drops at pH 5.6 by the vapour-diffusion method using 30% polyethylene glycol 4000 as precipitant. It belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 60.2, b = 76.6, c = 135.3 A. Reflections to 1.2 A resolution are observable. An initial atomic model with 472 residues has been built based on SeMet SAD data at 1.8 A resolution. Unexpectedly, the structure revealed a novel metal centre in the amidohydrolase superfamily.

机译：D-Aminoacylases催化的水解N-acyl-D-amino酸分子酸的锌离子的援助。水晶从粪产碱杆菌属获得在悬滴pH值5.6vapour-diffusion方法使用30%的聚乙烯4000年醇作为沉淀剂。集团P2(1) 2(1) 2(1),晶胞参数= 60.2, b = 76.6, c = 135.3。一项决议是可观测的。与472年残留是建立基于模型SeMet悲伤数据分辨率1.8。揭示了小说结构金属中心酰胺水解酶家族。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第9期|1482-1483|共2页
作者
Hsu CS; Chen SJ; Tsai YCLin TWLiaw SHWang AH;
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作者单位

Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan.;

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正文语种英语
中图分类化学;
关键词
Amidohydrolases; Arthrogryposis multiplex congenita, distal type 1; amide hydrolasesAnalog-digital conversionN-acyl-D-amino-acid deacylaseAlcaligenes faecalisAlcaligenesZINC IONSCrystallization;

机译：酰胺水解酶;关节弯曲多路复用congenita,远端1型;酰胺hydrolasesAnalog-digitalconversionN-acyl-D-amino-acidIONSCrystallization;

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Crystallization and preliminary crystallographic analysis of a D-aminoacylase from Alcaligenes faecalis DA1.

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