Preliminary X-ray crystallographic analysis of a soluble form of MntC, a periplasmic manganese-binding component of an ABC-type Mn transporter from Synechocystis sp. PCC 6803.

Adir N; Rukhman V; Brumshtein BAnati R

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preliminary X-ray crystallographic analysis of a soluble form of MntC, a periplasmic manganese-binding component of an ABC-type Mn transporter from Synechocystis sp. PCC 6803.

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Preliminary X-ray crystallographic analysis of a soluble form of MntC, a periplasmic manganese-binding component of an ABC-type Mn transporter from Synechocystis sp. PCC 6803.

机译：初步的x射线晶体分析可溶性MntC,周质的ABC-type Mn manganese-binding组件PCC 6803运输机从集胞藻属sp。

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摘要

Manganese is recruited in microorganisms by way of ABC-type transporter systems. Here, the expression, purification and preliminary crystallographic analysis of a soluble form of the MntC solute-binding protein component of the MntABC manganese-import system from the cyanobacterium Synechococystis sp. PCC 6803 is reported. The protein (321 amino-acid residues) was expressed exclusively in inclusion bodies, which required unfolding and refolding in the presence of manganese prior to purification. The purified protein was crystallized in the presence of PEG and zinc. The crystals belong to space group P6(2)22, with unit-cell parameters a = b = 128.1, c = 90.0 A and a single molecule in the asymmetric unit. The crystals diffract to 2.6 A under cryoconditions using synchrotron radiation.

机译：锰是微生物通过招募ABC-type转运系统。表达、纯化和初步的晶体的可溶性形式的分析的MntC solute-binding蛋白质的组成部分MntABC manganese-import系统的藻青菌Synechococystis sp, PCC 6803报道。只在包涵体表达,需要展开和折叠的吗净化前锰的存在。纯化蛋白结晶的存在的挂钩和锌。集团P6(2) 22日,晶胞参数a = b =128.1, c = 90.0和一个分子不对称单元。在cryoconditions使用同步加速器辐射。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第9期|1476-1478|共3页
作者
Adir N; Rukhman V; Brumshtein BAnati R;
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作者单位

Department of Chemistry and Institute of Catalysis, Science and Technology, Technion, Technion City, Haifa 32000, Israel. nadir@tx.technion.ac.il;

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正文语种英语
中图分类化学;
关键词
Periplasmic Binding Proteins; Periplasm; manganeseSynechocystis sp. PCC 6803SynechocystisCrystallization;

机译：周质的绑定6803年synechocystiscrystallization;

Preliminary X-ray crystallographic analysis of a soluble form of MntC, a periplasmic manganese-binding component of an ABC-type Mn transporter from Synechocystis sp. PCC 6803.

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