Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

Nina A. Brunner; Dietar A. Lang; Matthias WilmannsReinhard Hensel

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Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

机译：结晶和初步的x射线衍射分析NAD-dependent non-phosphorylating的GAPDH hyperthermophilic archaeon

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Recombinant non-phosphorylating NAD~+-dependent glyceralde-hyde-3-phosphate dehydrogenase (GAPN) of the hyperthermophilic crenarchaeote Thermoproteus tenax has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion technique. Crystals of different habits were obtained from several precipitant solutions (salts and polyethylene glycols). Preliminary X-ray analysis was performed with crystals grown in ammonium formate, which belonged to the primitive hexagonal space group P622, and had unit-cell parameters a = b = 184.8, c = 133.0 A, γ = 120°. Assuming a molecular weight of 55 kDa, a Matthews parameter of 3.3 A~3 Da~(-1) is calculated assuming two molecules per asymmetric unit. The diffraction limit of these crystals is 2.5 A resolution.

机译：重组non-phosphorylating NAD ~ +端依赖glyceralde-hyde-3-phosphate脱氢酶(GAPN)的hyperthermophilic crenarchaeoteThermoproteus tenax过表达,使用悬滴提纯和结晶vapour-diffusion技术。习惯了从几沉淀剂解决方案(盐和聚乙二醇)。初步的x射线分析甲酸铵晶体生长,属于原始的六角形空间群P622,晶胞参数a = b = 184.8,c = 133.0,γ= 120°。体重55 kDa,马修斯参数为3.3 ~ 3哒~(1)计算假设两个分子/不对称单元。晶体是2.5一项决议。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第1期|89-91|共3页
作者
Nina A. Brunner; Dietar A. Lang; Matthias WilmannsReinhard Hensel;
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EMBL Outstation Hamburg, c/o DESY, Notkestrasse 85, D-22038 Hamburg, Germany;

Fresenius Medical Care AG, Medical Department, Else-Kroener-Strasse 1, 61352 Bad Homburg, Germany;

FB 9 Mikrobiologie, Universitat-GH Essen, Universitatsstrasse 5, D-45117 Essen, Germany;

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Thermoproteus tenax; crystals; ammonium formateX-ray diffraction analysisCrystallizationhyperthermophilic archaeon;

机译：Thermoproteus tenax;晶体;formateX-ray铵衍射analysisCrystallizationhyperthermophilic archaeon;

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Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

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