Crystallization and preliminary X-ray diffraction studies of cytochrome c_6 from Porphyra yezoensis

Tomokazu Sasaki; Megumi Nakahara; Aya MatsudaTakenobu YamasakiSakae KatohShigeru OhshimaIsao OonishiAkira Uchida

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Crystallization and preliminary X-ray diffraction studies of cytochrome c_6 from Porphyra yezoensis

机译：结晶和初步的x射线衍射研究的细胞色素c_6 Porphyra yezoensis

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Cytochrome c_6 from the red alga Porphyra yezoensis has been purified and crystallized by the sitting-drop vapour-diffusion method. Two different crystal forms, tetragonal and orthorhombic, were obtained. The tetragonal crystals belong to space group P4_12_12 or P4_32_12, with unit-cell dimensions a = 49.33 (2), c = 83.70 (10) A. The orthorhombic crystals belong to space group P2_12_12_1, with unit-cell dimensions a = 46.74 (2), b = 49.42 (2), c = 37.11 (1) A. Absorption spectra of the crystals showed that the tetragonal form was oxidized and the orthorhombic form was reduced.

机译：从红藻细胞色素c_6 Porphyrayezoensis提纯和结晶的sitting-drop vapour-diffusion方法。不同的晶体形式,正方斜方晶系的获得。水晶属于组P4_12_12或空间P4_32_12,单胞尺寸= 49.33(2), c = 83.70(10)的斜方晶体属于空间群P2_12_12_1,晶胞维a = 46.74 (2), b = 49.42 (2), c =37.11(1)答:晶体的吸收光谱表明,正方被氧化和形式斜方晶系的形式被降低。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第1期|79-80|共2页
作者
Tomokazu Sasaki; Megumi Nakahara; Aya MatsudaTakenobu YamasakiSakae KatohShigeru OhshimaIsao OonishiAkira Uchida;
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作者单位

Department of Biology, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan;

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正文语种英语
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Porphyra yezoensis; Cytochromes; Cytochromes cX-ray diffractioncrystalscell dimensionsAbsorption spectraorthorhombic systemCrystallization;

机译：Porphyra yezoensis;细胞色素细胞色素cX-raydiffractioncrystalscell dimensionsAbsorptionspectraorthorhombic systemCrystallization;

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Crystallization and preliminary X-ray diffraction studies of cytochrome c_6 from Porphyra yezoensis

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