Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis

B. Gopal; R. A. Cox; M. J. ColstonG. G. DodsonS. J. SmerdonL. F. Haire

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Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis

机译：结晶和初步的x射线衍射研究N-utilizing物质b (NusB)从结核分枝杆菌

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N-utilizing substance B (NusB) is a protein which forms part of a complex assembly in transcriptional antitermination in Mycobacterium tuberculosis. It forms a heterodimer with the product of the NusE gene (identical to the ribosomal protein S10) and mediates the process of transcriptional antitermination by forming the core complex with the nut site of the ribosomal RNA along with other protein factors. NusB has been cloned and overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method. The space group is P2_12_12_1, with unit-cell parameters a = 46.6, b = 64.2, c = 90.1 A. A native data set complete to 1.6 A resolution has been collected from a single crystal.

机译：N-utilizing物质B (NusB)是一种蛋白质构成一个复杂的组装的一部分在分枝杆菌转录抗终止肺结核。NusE基因(相同的产物核糖体蛋白S10)和调节过程形成的转录抗终止核心的复杂与螺母的核糖体RNA与其他蛋白质的因素。被克隆并在大肠杆菌中使用悬滴和结晶vapour-diffusion方法。P2_12_12_1,晶胞参数= 46.6,b= 64.2, c = 90.1。1.6收集从一个决议晶体。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第1期|64-66|共3页
作者
B. Gopal; R. A. Cox; M. J. ColstonG. G. DodsonS. J. SmerdonL. F. Haire;
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作者单位

Division of Mycobacterial Research, The National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England;

Division of Protein Structure, The National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England;

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ribosomal protein S10; Mycobacterium tuberculosis; X-ray diffractionTranscription TerminationheterodimerRibosomal RNACrystallization;

机译：核糖体蛋白S10;分枝杆菌肺结核;x射线diffractionTranscriptionTerminationheterodimerRibosomalRNACrystallization;

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Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis

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