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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of full-length annexin I comprising the core and N-terminal domain
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Crystallization and preliminary X-ray analysis of full-length annexin I comprising the core and N-terminal domain

机译：结晶和初步的x射线分析我全身的膜联蛋白组成和核心n端结构域

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摘要

Annexin I, a member of the annexin family of Ca~(2+)- and phospholipid-binding proteins, has been crystallized with the complete N-terminus. Annexins are structurally divided into a conserved protein core and an N-terminal domain that is variable in sequence and length. Three-dimensional structures of annexins comprising the protein core and a short N-terminal domain (annexins III, IV, V, VI, XII) or a truncated form almost completely lacking the N-terminal domain (annexins I and II) have been published so far. Here, the crystallization of annexin I comprising not only the core but also the complete N-terminal domain is reported. The crystals belong to the space group P2_12_12_1, with unit-cell parameters a = 63.6, b = 96.3, c = 127.4 A, and diffract to better than 2 A. Assuming a molecular weight of 38.7 kDa for annexin I and an average value of 2.5 A~3 Da~(-1) for V_M, two molecules per asymmetric unit are present.
机译：膜联蛋白,膜联蛋白家族的一员Ca ~(2 +)和phospholipid-binding蛋白质,与完整的n端结晶。膜联蛋白结构分为守恒的蛋白质核心和一个n端结构域变量的顺序和长度。膜联蛋白的三维结构由蛋白质核心和一个短n端结构域(膜联蛋白III, IV, V, VI,十二)或截短形式几乎完全缺乏n端结构域(膜联蛋白I和II)迄今为止出版。膜联蛋白我不仅包括核心也完整的n端结构域。水晶属于空间群P2_12_12_1,晶胞参数= 63.6,b = 96.3, c =127.4,衍射比2。假设38.7 kDa的分子量膜联蛋白我和的平均值2.5 ~ 3哒~ (1)每个不对称单元V_M,两个分子礼物。

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