Preliminary crystallographic studies of an extremely thermostable KDG aldolase from Sulfolobus solfataricus

Elaine J. Hendry; Catriona L. Buchanan; Rupert J. M. RussellDavid W. HoughChristopher D. ReeveMichael J. DansonGarry L. Taylor

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Preliminary crystallographic studies of an extremely thermostable KDG aldolase from Sulfolobus solfataricus

机译：初步的晶体的研究极耐热性的KDG醛缩酶硫化叶菌solfataricus

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摘要

Crystals have been grown of 2-keto-3-deoxygluconate aldolase (KDG aldolase) from the hyperthermophilic archaeon Sulfolobus solfataricus that diffract to 2.2 A resolution. The enzyme catalyses the reversible aldol cleavage of 2-keto-3-dexoygluconate to pyruvate and glyceraldehyde, the third step of a modified non-phosphorylated Entner-Doudoroff pathway of glucose oxidation. S. solfataricus grows optimally at 353 K and the enzyme itself has a half-life of 2.5 h at 373 K. Knowledge of the crystal structure of KDG aldolase will further understanding of the basis of protein hyperthermo-stability and create a target for site-directed mutagenesis of activesite residues, with the aim of altering substrate specificity. Three crystal forms have been obtained: orthorhombic crystals of space group P2_12_12_1, which diffract to beyond 2.15 A, monoclinic crystals of space group C2, which diffract to 2.2 A, and cubic crystals of space group P4_232, which diffract to 3.4 A.

机译：晶体生长的从hyperthermophilic archaeon硫化叶菌solfataricus衍射分辨率2.2。酶催化作用可逆醇醛乳沟2-keto-3-dexoygluconate丙酮酸甘油醛,第三步的修改non-phosphorylated Entner-Doudoroff通路的葡萄糖氧化。最好在353 K和酶本身有一个半衰期为2.5 h在373 K。晶体结构的KDG醛缩酶将进一步了解蛋白质的基础hyperthermo-stability并创建一个目标定点诱变activesite残留物,目的是改变底物特异性。三个水晶形式取得了:斜方晶系的晶体的空间群P2_12_12_1,衍射超出2.15,单斜吗晶体的空间群C2,衍射到2.2一、立方晶体的空间群P4_232,衍射3.4。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第11期|1437-1439|共3页
作者
Elaine J. Hendry; Catriona L. Buchanan; Rupert J. M. RussellDavid W. HoughChristopher D. ReeveMichael J. DansonGarry L. Taylor;
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作者单位

Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, England;

Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland;

Avecia Life Science Molecules, Belasis Avenue, Billingham, Cleveland TS23 1YN, England;

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正文语种英语
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pyruvates; Sulfolobus solfataricus; crystal structureSite-Directed Mutagenesis2-keto-3-deoxy-D-glucarate aldolasecrystalsspace groups;

机译：structureSite-DirectedMutagenesis2-keto-3-deoxy-D-glucaratealdolasecrystalsspace组;

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2. SSoNA and SsoNAlong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus? [J] . UIGI MANDRIC, MARGHERITA PEZZULLO, MOSE ROSSIAND GIUSEPPE MANCO Archaea: an international microbiological journal . 2007,第2期

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3. Flt.-Lient. R. G. Veryard, B. Sc., R. A. F.: A Preliminary Study of a Toronado at Peshawar (トルネドー研究序報). India Met. Dep. Vol. V, No.56, 17th June. 1933. [J] . 佐々木孝一 ournal of the Meteorological Society of Japan . 1934,第11期

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4. SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation. [J] . Albers SV, Birkeland NK, Driessen AJGertig SHaferkamp PKlenk HPKouril TManica APham TKRuoff PSchleper CSchomburg DSharkey KJSiebers BSierocinski PSteuer Rvan-der-Oost JWesterhoff HVWieloch PWright PCZaparty M Biochemical Society Transactions . 2009,第Pta1期

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Preliminary crystallographic studies of an extremely thermostable KDG aldolase from Sulfolobus solfataricus

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