Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

Joao M. Dias; Carlos A. Cunha; Susana TeixeiraGabriela AlmeidaCristina CostaJorge LampreiaJose J. G. MouraIsabel MouraMaria Joao Romao

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

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Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

机译：结晶和初步的x射线分析一个包围的亚硝酸还原酶脱磷孤菌属desulfuricans写明ATCC 27774

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Nitrite reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is a multihaem (type c) membrane-bound enzyme that catalyzes the dissimilatory conversion of nitrite to ammonia. Crystals of the oxidized form of this enzyme were obtained using PEG and CaCl_2 as precipitants in the presence of 3-(decyl-methylammonium)propane-1-sulfonate and belong to the space group P2_12_12_1, with unit-cell parameters a = 78.94, b = 104.59, c = 143.18 A. A complete data set to 2.30 A resolution was collected using synchrotron radiation at the ESRF. However, the crystals may diffract to beyond 1.7 A and high-resolution data will be collected in the near future.

机译：从硫酸盐还原亚硝酸盐还原酶细菌脱磷孤菌属desulfuricans写明ATCC 27774是一个multihaem (c型)膜结合酶催化的异化的转换亚硝酸氨。这种酶得到使用和CaCl_2挂钩沉淀剂的存在(3) - decyl-methylammonium propane-1-sulfonate和属于空间群P2_12_12_1,晶胞参数= 78.94,b = 104.59, c =143.18。使用同步收集的决议欧洲同步辐射实验室的辐射。衍射超出1.7和高分辨率数据将在不久的将来。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第2期|215-217|共3页
作者
Joao M. Dias; Carlos A. Cunha; Susana TeixeiraGabriela AlmeidaCristina CostaJorge LampreiaJose J. G. MouraIsabel MouraMaria Joao Romao;
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作者单位

Departamento de Quimica, Centro de Quimica Fina e Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2825-114 Monte de Caparica, Portugal;

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Nitrite Reductases; Desulfovibrio desulfuricans; X-ray analysissynchrotron radiationEnzymesCrystallization;

机译：亚硝酸盐还原酶、脱磷孤菌属desulfuricans;x射线analysissynchrotronradiationEnzymesCrystallization;

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1. Science Letters:Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii [J] . GAO Yong-gui, YAO Min, TANAKA Isao 浙江大学学报（英文版）（B辑：生物医学和生物技术） . 2005,第006期
2. In the facultative sulphateitrate reducer Desulfovibrio desulfuricans ATCC 27774, the nine-haem, cytochrome c is part of a membrane-bound redox complex mainly expressed in sulphate-grown cells [J] . Ligia M. Saraiva, Patricia N. da Costa, Cristiano Conte, Biochimica et biophysica acta. Gene structure and expression . 2001,第1期

机译：在兼性硫酸盐/硝酸盐还原剂Desulfovibrio desulfuricans ATCC 27774中，九个血红细胞色素c是膜结合的氧化还原复合物的一部分，主要在硫酸盐生长的细胞中表达
3. [NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774: gene sequencing, three-dimensional structure determination and refinement at 1.8 A and modelling studies of its interaction with the tetrahaem cytochrome c_3 [J] . Pedro M. Matias, Claudio M. Soares, Ligia M. Saraiva, Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry . 2001,第1期

机译：Desulfovibrio desulfuricans ATCC 27774的[NiFe]氢化酶：基因测序，三维结构确定和1.8 A精制及其与四色细胞色素c_3相互作用的模型研究
4. The influence of the desulfovibrio desulfuricans 14 ATCC 27774 on the corrosion of mild steel [J] . M. J. Feio, V. Rainha, M. A. Reis, Materials and Corrosion . 2000,第10期

机译：desulfovibrio desulfuricans 14 ATCC 27774对低碳钢腐蚀的影响
5. Mossbauer study of hexaheme nitrite reductase from Desulfovibrio desulfuricans (ATCC 27774). [D] . Wang, Yaning. 1991

机译：Mossbauer研究了Desulfovibrio desulfuricans（ATCC 27774）中的亚硝酸六血红素还原酶。
6. Purification crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 [O] . Olga Yu. Gavel, Anna V. Kladova, Sergey A. Bursakov, 2008

机译：硫酸盐还原菌Desulfovibrio desulfuricans ATCC 27774的三磷酸腺苷三磷酸腺苷化酶（ATPS）的纯化结晶和初步X射线衍射分析
7. Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom [O] . Chou, C.C., Hou, M.H., 侯明宏 2014

机译：Crystallization and preliminary X-ray diffraction analysis of phospholipase a(1) isolated from hornet (Vespa basalis) venom

Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

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