Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

Lars K. Skov; Osman Mirza; Anette HenriksenGabrielle Potocki De MontalkMagali Remaud-SimeonPatricia SarcabalRene-Marc WillemotPierre MonsanMichael Gajhede

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Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

机译：结晶和初步的x射线的研究重组amylosucrase从奈瑟氏菌属

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Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2_12_12, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 A, and diffract to 1.6 A resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.

机译：重组amylosucrase从奈瑟氏菌属polysaccharea结晶了vapour-diffusion过程的存在聚乙二醇6000。斜方晶系的空间群P2_12_12,晶胞参数= 95.7,b = 117.2, c =62.1, 1.6和衍射分辨率。p-chloromercuribenzene磺酸盐(pcmbs)已被确认和导数selenomethionine-substituted蛋白质已经生产和结晶。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第2期|203-205|共3页
作者
Lars K. Skov; Osman Mirza; Anette HenriksenGabrielle Potocki De MontalkMagali Remaud-SimeonPatricia SarcabalRene-Marc WillemotPierre MonsanMichael Gajhede;
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作者单位

Institut National des Sciences Appliquees, Avenue de Rangueil, F-31077 Toulouse CEDEX 4, France;

Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark;

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正文语种英语
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amylosucrase; Neisseria polysaccharea; genetic modificationX-raysCrystallization;

机译：modificationX-raysCrystallization;

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Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

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