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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Improved crystals of Thermus thermophilus prolyl-tRNA synthetase complexed with cognate tRNA obtained by crystallization from precipitate
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Improved crystals of Thermus thermophilus prolyl-tRNA synthetase complexed with cognate tRNA obtained by crystallization from precipitate

机译：改善晶体栖热菌属的酸奶prolyl-tRNA合成酶与同源获得的tRNA结晶沉淀

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摘要

The complex between Thermus thermophilus prolyl-tRNA synthetase (ProRSTT) and its cognate tRNA has been crystallized using two different isoacceptors of tRNA~(Pro). Similar bipyramidal crystals of the complexes of ProRSTT with the two different tRNA~(Pro) isoacceptors grow within two weeks from 32% saturated ammonium sulfate solution. They belong to space group P4_32_12, with unit-cell parameters a = 143.1, b = 143.1, c = 228.6 A. The crystals diffract weakly to a maximum resolution of 3.1 A. Superior quality crystals were obtained by growing slowly from precipitate over 5-6 months. These are of the same space group but have slightly altered unit-cell parameters, a = 140.8, b = 140.8, c = 237.0 A. These crystals diffract more strongly to at least 2.8 A resolution and a complete data set to 2.85 A resolution has been collected from a single crystal. Comparison of the packing in the two crystal forms shows that domain flexibility contributes to the presence of different crystal contacts in the two forms.
机译：栖热菌属之间的复杂的酸奶prolyl-tRNA合成酶(ProRSTT)及其同源tRNA一直使用两个不同的结晶isoacceptors tRNA ~ (Pro)。复合物的晶体ProRSTT两个不同的tRNA ~ (Pro) isoacceptors成长在两周从32%饱和硫酸铵解决方案。晶胞参数= 143.1,= 143.1 b, c= 228.6。3.1的最大分辨率。得到了晶体的增长缓慢沉淀在5 - 6个月。相同的空间群但略有改变晶胞参数= 140.8,b = 140.8, c =237.0。至少2.8分辨率和一个完整的数据集2.85决议已经收集到的单晶。两种晶体形式显示域的灵活性有助于不同晶体的存在接触的两种形式。

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