1.7 A X-ray structure of space-grown collagenase crystals

I. Broutin-LHermite; M. Ries-Kautt; A. DucruixLaboratoire dEnzymologie et de Biochimie Structurales Batiment 34 CNRS 91198 Gif sur Yvette CEDEX France

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1.7 A X-ray structure of space-grown collagenase crystals

机译：1.7 x射线结构空间长大胶原酶晶体

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Collagenases, divided into metallocollagenases and serine collagenases, are the only proteases that cleave the triple helix of collagen under physiological conditions. In the present work, the serine protease collagenase purified from Hypoderma lineatum larvae is studied. From crystals grown in the International Microgravity Laboratory (IML2), a data set was collected at 1.7 A using synchrotron radiation. Although the resolution is not very different, the signal-to-noise ratio and the quality of the electron density are much improved. Alternate conformations were revealed for several residues, in particular Tyr99, suggesting a gate mechanism of recognition.

机译：胶原酶,分为metallocollagenases和丝氨酸胶原酶,是唯一的蛋白酶裂开的胶原蛋白三螺旋生理条件。丝氨酸蛋白酶的纯化胶原酶真皮lineatum幼虫进行了研究。在国际微重力晶体生长实验室(IML2),一个数据集收集1.7使用同步加速器辐射。分辨率不是很不同,信噪比和质量电子密度就会大大提高。构象透露了一些残留物,特别是Tyr99,暗示闸门机制的认可。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第3期|376-378|共3页
作者
I. Broutin-LHermite; M. Ries-Kautt; A. DucruixLaboratoire dEnzymologie et de Biochimie Structurales Batiment 34 CNRS 91198 Gif sur Yvette CEDEX France;
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Laboratoire d'Enzymologie et de Biochimie Structurales, Batiment 34, CNRS, 91198 Gif sur Yvette CEDEX, France;

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正文语种英语
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Collagenases; Hypoderma lineatum; ResiduesCollagenElectron densitytriple helixcrystalssynchrotron radiationResolving powerX RAY STRUCTUREDataset;

机译：Collagenases;Hypodermalineatum;ResiduesCollagenElectron densitytriplehelixcrystalssynchrotron radiationResolvingpowerX射线STRUCTUREDataset;

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1.7 A X-ray structure of space-grown collagenase crystals

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