Crystallization and preliminary X-ray diffraction analysis of β-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin

M. Archer; M. L. Rodrigues; M. AurelioR. BiemansA. CravadorM. A. Carrondo

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Crystallization and preliminary X-ray diffraction analysis of β-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin

机译：结晶和初步的x射线衍射分析β-cinnamomin, elicitin分泌植物病原真菌的疫霉cinnamomin

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Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapour-diffusion method using either ammonium sulfate or polyethylene-glycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A, α = 76.86, β = 84.41, γ = 80.26°. A frozen crystal diffracted X-rays beyond 1.45 A resolution on a synchrotron-radiation source.

机译：Cinnamomin (CIN) 10 kDa的属于一个家庭指定为elicitins的蛋白质。蛋白产生过敏的反应不同的植物物种,导致阻力植物病原真菌对真菌和细菌。由vapour-diffusion结晶方法用硫酸铵或聚乙二醇(PEG)作为沉淀剂解决方案缓冲pH值约为7。同晶型,属于三斜晶系的空间b组,晶胞参数= 31.69,=80.26°。1.45解决电脑同步源。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第3期|363-365|共3页
作者
M. Archer; M. L. Rodrigues; M. AurelioR. BiemansA. CravadorM. A. Carrondo;
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作者单位

Unidade de Ciencias e Tecnologias Agrarias, Universidade do Algarve, Campus de Gambelas, 8000-810 Faro, Portugal;

Instituto de Tecnologia Quimica e Biologica (ITQB), Av. Republica, Apt 127, 2781-901 Oeiras, Portugal;

Service de Genetique Appliquee, Universite Libre de Bruxelles, 1400 Nivelles, Belgium;

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正文语种英语
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elicitin; Hypersensitive response; Synchrotron radiation sourcesplant pathogenic fungicinnamominspace groupsplant speciesCrystallizationisomorphous substitutionPhytophthoraammonium sulfateFungiX-ray diffraction analysis;

机译：elicitin;高度敏感反应;同步辐射sourcesplant致病性fungicinnamominspace groupsplantspeciesCrystallizationisomorphoussubstitutionPhytophthoraammoniumsulfateFungiX-ray衍射分析;

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3. Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein [J] . Huet Jean-Claude, Pernollet Jean-Claude FEBS Letters . 1989,第2期

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Crystallization and preliminary X-ray diffraction analysis of β-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin

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