Crystallization and preliminary crystallographic analysis of Thermus thermophilus leucyl-tRNA synthetase and its complexes with leucine and a non-hydrolysable leucyl-adenylate analogue

Anna Yaremchuk; Stephen Cusack; Olga GudzeraMorten GrotliMikhail Tukalo

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Crystallization and preliminary crystallographic analysis of Thermus thermophilus leucyl-tRNA synthetase and its complexes with leucine and a non-hydrolysable leucyl-adenylate analogue

机译：结晶和初步的晶体分析栖热菌属酸奶leucyl-tRNA合成酶及其复合物亮氨酸和non-hydrolysable leucyl-adenylate模拟

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Leucyl-tRNA synthetase from Thermus thermophilus (LeuRSTT) is the first LeuRS to be crystallized. Two crystal forms of the native enzyme have been obtained using the hanging-drop vapour-diffusion method with ammonium sulfate as a precipitant. Crystals of the first form belong to space group I422 and have unit-cell parameters a = b = 312.4, c = 100.4 A. They diffract anisotropically to 3.5 A resolution in the c-axis direction and to only 6 A resolution in the perpendicular direction. Crystals of the second form, which can be obtained native or with leucine or a leucyl-adenylate analogue bound, belong to space group C222_1 and have unit-cell parameters a = 102.4, b = 154.1, c = 174.3 A. They diffract to 1.9 A resolution and contain one monomer in the asymmetric unit. Selenomethionated LeuRSTT has been produced and crystals of the second form suitable for MAD analysis have been grown.

机译：从栖热菌属Leucyl-tRNA合成酶酸奶(LeuRSTT)是第一个他们的结晶。两种晶体形式的原生酶获得使用悬滴vapour-diffusion方法用硫酸铵沉淀剂。晶体的第一种形式属于空间群I422,晶胞参数a = b = 312.4,c = 100.4。c-axis方向,只有一项决议6垂直方向的一项决议。第二种形式的晶体,可以获得本地或亮氨酸和leucyl-adenylate模拟绑定,属于空间组C222_1,晶胞参数=102.4, b = 154.1, c = 174.3。分辨率和1.9包含一个单体不对称单元。第二种形式的生产和晶体适用于疯狂的分析一直在增长。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第5期|667-669|共3页
作者
Anna Yaremchuk; Stephen Cusack; Olga GudzeraMorten GrotliMikhail Tukalo;
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作者单位

Center for Solid Phase Organic Combinatorial Chemistry, Calsberg Laboratory, Gamle Calsberg Vej 10, DK-2300 Valby, Denmark;

EMBL, Grenoble Outstation, c/o ILL, 6 Rue Jules Horowitz, F-38042 Grenoble CEDEX 9, France;

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 252627, Kiev 143, Ukraine;

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Leucine; cell parameter; ammonium sulfateThermus thermophiluscrystalsC-axisAnisotropic magnetoresistanceAmino Acyl-tRNA SynthetasescomplexesLeucine-tRNA LigaseCrystallization;

机译：亮氨酸;细胞参数;sulfateThermus铵thermophiluscrystalsC-axisAnisotropicmagnetoresistanceAmino Acyl-tRNASynthetasescomplexesLeucine-tRNA;

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Crystallization and preliminary crystallographic analysis of Thermus thermophilus leucyl-tRNA synthetase and its complexes with leucine and a non-hydrolysable leucyl-adenylate analogue

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