Cloning, expression, purification and crystallization of saccharopine reductase from Magnaporthe grisea

Eva Johansson; James J. Steffens; Mark EmptageYlva LindqvistGunter Schneider

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Cloning, expression, purification and crystallization of saccharopine reductase from Magnaporthe grisea

机译：克隆、表达、纯化和结晶的saccharopine还原酶稻瘟病菌

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The gene coding for saccharopine reductase (E.C. 1.5.1.10), an enzyme of the α-aminoadipic pathway of lysine biosynthesis in the pathogenic fungus Magnaporthe grisea, was cloned and expressed in Escherichia coli. The purified enzyme was crystallized in space groups C2 and C222_1 using ammonium sulfate pH 4.8 or PEG 6000 pH 4.1 as precipitants. The unit-cell parameters are a = 115.0, b = 56.6, c = 74.3 A, β = 111.1° for space group C2, and a = 89.3, b = 119.0, c = 195.9 A for space group C222_1. The crystals diffract to resolutions of 2.0 A (C2) and 2.4 A (C222_1) at synchrotron sources.

机译：saccharopine还原酶基因编码(提到过1.5.1.10),一种酶α-aminoadipic途径赖氨酸生物合成的病原真菌稻瘟病菌,克隆和表达大肠杆菌。结晶在太空组C2和C222_1使用硫酸铵酸碱盯住4.8或6000 4.1沉淀剂。115.0, b = 56.6, c = 74.3,β= 111.1°的空间C2组,b = 89.3, = 119.0, c = 195.9空间群C222_1。决议(C2) 2.0和2.4 (C222_1)同步源。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第5期|662-664|共3页
作者
Eva Johansson; James J. Steffens; Mark EmptageYlva LindqvistGunter Schneider;
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作者单位

Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden;

Central Research and Development, EI Du Pont de Nemours and Co., Experimental Station, Wilmington, DE 19880-0328, USA;

DuPont Agricultural Products, Stine-Haskell Ressearch Center, PO Box 30, Newark, DE 19714, USA;

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正文语种英语
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Escherichia coli; Purification; space groupsMagnaporthe griseaCloningLysine Biosynthesis PathwayCrystallization;

机译：大肠杆菌;净化;空间生物合成PathwayCrystallization;

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Cloning, expression, purification and crystallization of saccharopine reductase from Magnaporthe grisea

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