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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification and crystallization of Enterococcus faecium streptogramin A acetyltransferase
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Expression, purification and crystallization of Enterococcus faecium streptogramin A acetyltransferase

机译：的表达、纯化和结晶肠球菌都有效streptogramin一乙酰转移酶

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摘要

The streptogramin A acetyltransferase from Enterococcus faecium (SWISS-PROT P50870) has been overexpressed in Escherichia coli, purified and crystallized. Crystallization conditions were screened using the hanging-drop vapor-diffusion method and resulted in two distinct crystal forms. Form I crystals diffract to 2.5 A and belong to space group P2_12_12_1, with unit-cell parameters a = 68.6, b = 102.6, c = 107.5 A. Form II crystals diffract to 2.7 A and belong to space group F222, with unit-cell parameters a = 185.8, b = 185.8, c = 186.5 A. Rotation-function and packing analyses for both crystal forms indicate that the asymmetric unit may contain one and two copies of the trimeric enzyme for crystal forms I and II, respectively.
机译：streptogramin乙酰转移酶从肠球菌都有效(SWISS-PROT P50870)在大肠杆菌中,纯化和结晶。筛选使用悬滴扩散方法和导致了两种截然不同的晶体形式。属于空间群P2_12_12_1,晶胞参数= 68.6,b = 102.6, c = 107.5。2.7二世晶体衍射和属于空间集团F222,晶胞参数= 185.8,b = 185.8, c = 186.5。包装对晶体形式显示分析不对称单元可以包含1和2我的副本三聚物的酶晶体形式第二,分别。

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